A Common Variant in CLDN14 is Associated with Primary Biliary Cirrhosis and Bone Mineral Density

Ruqi Tang, Yiran Wei, Zhiqiang Li, Haoyan Chen, Qi Miao, Zhaolian Bian, Haiyan Zhang, Qixia Wang, Zhaoyue Wang, Min Lian, Fan Yang, Xiang Jiang, Yue Yang, Enling Li, Michael F Seldin, M. Eric Gershwin, Wilson Liao, Yongyong Shi, Xiong Ma

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Abstract

Primary biliary cirrhosis (PBC), a chronic autoimmune liver disease, has been associated with increased incidence of osteoporosis. Intriguingly, two PBC susceptibility loci identified through genome-wide association studies are also involved in bone mineral density (BMD). These observations led us to investigate the genetic variants shared between PBC and BMD. We evaluated 72 genome-wide significant BMD SNPs for association with PBC using two European GWAS data sets (n = 8392), with replication of significant findings in a Chinese cohort (685 cases, 1152 controls). Our analysis identified a novel variant in the intron of the CLDN14 gene (rs170183, Pfdr = 0.015) after multiple testing correction. The three associated variants were followed-up in the Chinese cohort; one SNP rs170183 demonstrated consistent evidence of association in diverse ethnic populations (Pcombined = 2.43 × 10-5). Notably, expression quantitative trait loci (eQTL) data revealed that rs170183 was correlated with a decline in CLDN14 expression in both lymphoblastoid cell lines and T cells (Padj = 0.003 and 0.016, respectively). In conclusion, our study identified a novel PBC susceptibility variant that has been shown to be strongly associated with BMD, highlighting the potential of pleiotropy to improve gene discovery.

Original languageEnglish (US)
Article number19877
JournalScientific Reports
Volume6
DOIs
StatePublished - Feb 4 2016

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Biliary Liver Cirrhosis
Bone Density
Genome-Wide Association Study
Single Nucleotide Polymorphism
Quantitative Trait Loci
Genetic Association Studies
Introns
Autoimmune Diseases
Osteoporosis
Liver Diseases
Genome
T-Lymphocytes
Cell Line
Incidence
Population
Genes

ASJC Scopus subject areas

  • General

Cite this

A Common Variant in CLDN14 is Associated with Primary Biliary Cirrhosis and Bone Mineral Density. / Tang, Ruqi; Wei, Yiran; Li, Zhiqiang; Chen, Haoyan; Miao, Qi; Bian, Zhaolian; Zhang, Haiyan; Wang, Qixia; Wang, Zhaoyue; Lian, Min; Yang, Fan; Jiang, Xiang; Yang, Yue; Li, Enling; Seldin, Michael F; Gershwin, M. Eric; Liao, Wilson; Shi, Yongyong; Ma, Xiong.

In: Scientific Reports, Vol. 6, 19877, 04.02.2016.

Research output: Contribution to journalArticle

Tang, R, Wei, Y, Li, Z, Chen, H, Miao, Q, Bian, Z, Zhang, H, Wang, Q, Wang, Z, Lian, M, Yang, F, Jiang, X, Yang, Y, Li, E, Seldin, MF, Gershwin, ME, Liao, W, Shi, Y & Ma, X 2016, 'A Common Variant in CLDN14 is Associated with Primary Biliary Cirrhosis and Bone Mineral Density', Scientific Reports, vol. 6, 19877. https://doi.org/10.1038/srep19877
Tang, Ruqi ; Wei, Yiran ; Li, Zhiqiang ; Chen, Haoyan ; Miao, Qi ; Bian, Zhaolian ; Zhang, Haiyan ; Wang, Qixia ; Wang, Zhaoyue ; Lian, Min ; Yang, Fan ; Jiang, Xiang ; Yang, Yue ; Li, Enling ; Seldin, Michael F ; Gershwin, M. Eric ; Liao, Wilson ; Shi, Yongyong ; Ma, Xiong. / A Common Variant in CLDN14 is Associated with Primary Biliary Cirrhosis and Bone Mineral Density. In: Scientific Reports. 2016 ; Vol. 6.
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abstract = "Primary biliary cirrhosis (PBC), a chronic autoimmune liver disease, has been associated with increased incidence of osteoporosis. Intriguingly, two PBC susceptibility loci identified through genome-wide association studies are also involved in bone mineral density (BMD). These observations led us to investigate the genetic variants shared between PBC and BMD. We evaluated 72 genome-wide significant BMD SNPs for association with PBC using two European GWAS data sets (n = 8392), with replication of significant findings in a Chinese cohort (685 cases, 1152 controls). Our analysis identified a novel variant in the intron of the CLDN14 gene (rs170183, Pfdr = 0.015) after multiple testing correction. The three associated variants were followed-up in the Chinese cohort; one SNP rs170183 demonstrated consistent evidence of association in diverse ethnic populations (Pcombined = 2.43 × 10-5). Notably, expression quantitative trait loci (eQTL) data revealed that rs170183 was correlated with a decline in CLDN14 expression in both lymphoblastoid cell lines and T cells (Padj = 0.003 and 0.016, respectively). In conclusion, our study identified a novel PBC susceptibility variant that has been shown to be strongly associated with BMD, highlighting the potential of pleiotropy to improve gene discovery.",
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