A combined hiPSC-derived endothelial cell and in vitro microfluidic platform for assessing biomaterial-based angiogenesis

Sylvia L. Natividad-Diaz, Shane Browne, Amit K. Jha, Zhen Ma, Samir Hossainy, Yosuke K. Kurokawa, Steven George, Kevin E. Healy

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Human induced pluripotent stem cell (hiPSC) derived angiogenesis models present a unique opportunity for patient-specific platforms to study the complex process of angiogenesis and the endothelial cell response to biomaterial and biophysical changes in a defined microenvironment. We present a refined method for differentiating hiPSCs into a CD31 + endothelial cell population (hiPSC-ECs) using a single basal medium from pluripotency to the final stage of differentiation. This protocol produces endothelial cells that are functionally competent in assays following purification. Subsequently, an in vitro angiogenesis model was developed by encapsulating the hiPSC-ECs into a tunable, growth factor sequestering hyaluronic acid (HyA) matrix where they formed stable, capillary-like networks that responded to environmental stimuli. Perfusion of the networks was demonstrated using fluorescent beads in a microfluidic device designed to study angiogenesis. The combination of hiPSC-ECs, bioinspired hydrogel, and the microfluidic platform creates a unique testbed for rapidly assessing the performance of angiogenic biomaterials.

Original languageEnglish (US)
Pages (from-to)73-83
Number of pages11
JournalBiomaterials
Volume194
DOIs
StatePublished - Feb 1 2019

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Induced Pluripotent Stem Cells
Microfluidics
Endothelial cells
Biocompatible Materials
Stem cells
Biomaterials
Endothelial Cells
Lab-On-A-Chip Devices
Hyaluronic acid
Hydrogel
Hyaluronic Acid
Testbeds
Hydrogels
Purification
Assays
Intercellular Signaling Peptides and Proteins
Perfusion
Network protocols
In Vitro Techniques
Population

Keywords

  • Differentiation
  • hiPSC-derived endothelial cells (hiPSC-ECs)
  • Human induced pluripotent stem cells (hiPSCs)
  • Hyaluronic acid
  • Hydrogel
  • In vitro angiogenesis model

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

Cite this

Natividad-Diaz, S. L., Browne, S., Jha, A. K., Ma, Z., Hossainy, S., Kurokawa, Y. K., ... Healy, K. E. (2019). A combined hiPSC-derived endothelial cell and in vitro microfluidic platform for assessing biomaterial-based angiogenesis. Biomaterials, 194, 73-83. https://doi.org/10.1016/j.biomaterials.2018.11.032

A combined hiPSC-derived endothelial cell and in vitro microfluidic platform for assessing biomaterial-based angiogenesis. / Natividad-Diaz, Sylvia L.; Browne, Shane; Jha, Amit K.; Ma, Zhen; Hossainy, Samir; Kurokawa, Yosuke K.; George, Steven; Healy, Kevin E.

In: Biomaterials, Vol. 194, 01.02.2019, p. 73-83.

Research output: Contribution to journalArticle

Natividad-Diaz, Sylvia L. ; Browne, Shane ; Jha, Amit K. ; Ma, Zhen ; Hossainy, Samir ; Kurokawa, Yosuke K. ; George, Steven ; Healy, Kevin E. / A combined hiPSC-derived endothelial cell and in vitro microfluidic platform for assessing biomaterial-based angiogenesis. In: Biomaterials. 2019 ; Vol. 194. pp. 73-83.
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