A cluster analysis of bacterial vaginosis-associated microflora and pelvic inflammatory disease

Roberta B. Ness, Kevin E. Kip, Sharon L. Hillier, David E. Soper, Carol A. Stamm, Richard L Sweet, Peter Rice, Holly E. Richter

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168 Scopus citations

Abstract

Controversy surrounds the association between bacterial vaginosis (BV) and pelvic inflammatory disease (PID). Women (N = 1,140) were ascertained at five US centers, enrolled (1999-2001), and followed up for a median of 3 years. Serial vaginal swabs were obtained for Gram's stain and cultures. PID was defined as 1) histologic endometritis or 2) pelvic pain and tenderness plus oral temperature >38.8°C, leukorrhea or mucopus, erythrocyte sedimentation rate >15 mm/hour, white blood cell count >10,000, or gonococcal/chlamydial lower genital infection. Exploratory factor analysis identified two discrete clusters of genital microorganisms. The first correlated with BV by Gram's stain and consisted of the absence of hydrogen peroxide-producing lactobacillus, Gardnerella vaginalis, Mycoplasma hominis, anaerobic Gram-negative rods, and, to a lesser degree, Ureaplasma urealyticum. The second, unrelated to BV by Gram's stain, consisted of Enterococcus species and Escherichia coli. Being in the highest tertile in terms of growth of BV-associated microorganisms increased PID risk (adjusted rate ratio = 2.03, 95% confidence interval: 1.16, 3.53). Carriage of non-BV-associated microorganisms did not increase PID risk. Women with heavy growth of BV-associated microorganisms and a new sexual partner appeared to be at particularly high risk (adjusted rate ratio = 8.77, 95% confidence interval: 1.11, 69.2). When identified by microbial culture, a combination of BV-related microorganisms significantly elevated the risk of acquiring PID.

Original languageEnglish (US)
Pages (from-to)585-590
Number of pages6
JournalAmerican Journal of Epidemiology
Volume162
Issue number6
DOIs
StatePublished - Sep 2005

Keywords

  • Chlamydia
  • Gonorrhea
  • Pelvic inflammatory disease
  • Sexually transmitted disease
  • Vaginitis

ASJC Scopus subject areas

  • Epidemiology

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    Ness, R. B., Kip, K. E., Hillier, S. L., Soper, D. E., Stamm, C. A., Sweet, R. L., Rice, P., & Richter, H. E. (2005). A cluster analysis of bacterial vaginosis-associated microflora and pelvic inflammatory disease. American Journal of Epidemiology, 162(6), 585-590. https://doi.org/10.1093/aje/kwi243