Abstract
In a clonal analysis of the IgE response, it was found that a small proportion of primary or nonimmune B cells in spleen and mesenteric lymph nodes can be stimulated by antigen to produce IgE-secreting clones. In addition, there appears to be no substantial difference in the frequency of such cells between the classical low and high IgE responder strains. An analysis of immune, or memory, B cells revealed substantial increases in the frequency of B cells secreting IgE as compared with primary B cells, although the actual proportion of B cells secreting IgE remained relatively low. When the IgE-secreting clones derived from either primary or secondary B cells were reanalyzed for the presence of other isotypes, it was found that all clones secreting IgE were secreting at least one other isotype, with the majority secreting two or three other isotypes in addition to IgE. This demonstrates that there is no distinct subpopulation of B cells committed to IgE expression per se.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 783-792 |
| Number of pages | 10 |
| Journal | Journal of Experimental Medicine |
| Volume | 153 |
| Issue number | 4 |
| State | Published - 1981 |
Fingerprint
ASJC Scopus subject areas
- Immunology
Cite this
A clonal analysis of the IgE response and its implications with regard to isotope commitment. / Teale, J. M.; Liu, Fu-Tong; Katz, D. H.
In: Journal of Experimental Medicine, Vol. 153, No. 4, 1981, p. 783-792.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A clonal analysis of the IgE response and its implications with regard to isotope commitment
AU - Teale, J. M.
AU - Liu, Fu-Tong
AU - Katz, D. H.
PY - 1981
Y1 - 1981
N2 - In a clonal analysis of the IgE response, it was found that a small proportion of primary or nonimmune B cells in spleen and mesenteric lymph nodes can be stimulated by antigen to produce IgE-secreting clones. In addition, there appears to be no substantial difference in the frequency of such cells between the classical low and high IgE responder strains. An analysis of immune, or memory, B cells revealed substantial increases in the frequency of B cells secreting IgE as compared with primary B cells, although the actual proportion of B cells secreting IgE remained relatively low. When the IgE-secreting clones derived from either primary or secondary B cells were reanalyzed for the presence of other isotypes, it was found that all clones secreting IgE were secreting at least one other isotype, with the majority secreting two or three other isotypes in addition to IgE. This demonstrates that there is no distinct subpopulation of B cells committed to IgE expression per se.
AB - In a clonal analysis of the IgE response, it was found that a small proportion of primary or nonimmune B cells in spleen and mesenteric lymph nodes can be stimulated by antigen to produce IgE-secreting clones. In addition, there appears to be no substantial difference in the frequency of such cells between the classical low and high IgE responder strains. An analysis of immune, or memory, B cells revealed substantial increases in the frequency of B cells secreting IgE as compared with primary B cells, although the actual proportion of B cells secreting IgE remained relatively low. When the IgE-secreting clones derived from either primary or secondary B cells were reanalyzed for the presence of other isotypes, it was found that all clones secreting IgE were secreting at least one other isotype, with the majority secreting two or three other isotypes in addition to IgE. This demonstrates that there is no distinct subpopulation of B cells committed to IgE expression per se.
UR - http://www.scopus.com/inward/record.url?scp=0019462661&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0019462661&partnerID=8YFLogxK
M3 - Article
C2 - 6972990
AN - SCOPUS:0019462661
VL - 153
SP - 783
EP - 792
JO - Journal of Experimental Medicine
JF - Journal of Experimental Medicine
SN - 0022-1007
IS - 4
ER -