A clomazone immunoassay to study the environmental fate of the herbicide in rice (Oryza sativa) agriculture

Mariana Carlomagno, Cecilia Mathó, Guillermina Cantou, James R. Sanborn, Jerold A Last, Bruce D. Hammock, Alvaro Roel, David González, Gualberto González-Sapienza

Research output: Contribution to journalArticlepeer-review

6 Scopus citations


The environmental impact of rice agriculture is poorly studied in developing countries, mainly due to limitations of the analytical capacity. Here, we report the development of a clomazone enzyme-linked immunosorbent assay as a fast and cost-effective tool to monitor the dissipation of this herbicide along the harvest. Antibodies were prepared using different strategies of hapten conjugation, and the best hapten/antibody pair was selected. It proved to be a reliable tool to measure the herbicide in the 2.0?20 ng/mL range in field samples, with excellent correlation with high-performance liquid chromatography results. The assay was used to study the dissipation of the herbicide in the floodwater of experimental rice paddies in Uruguay. Large differences in the residual amounts of herbicide were observed depending on the flooding practices. Because of its robustness and simplicity, the assay may be useful to delineate and monitor management practices that can contribute to minimizing the release of the herbicide in the environment.

Original languageEnglish (US)
Pages (from-to)4367-4371
Number of pages5
JournalJournal of Agricultural and Food Chemistry
Issue number7
StatePublished - Apr 14 2010


  • Clomazone
  • HPLC
  • Oryza sativa
  • Rice

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Chemistry(all)


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