A CHO-cell mutant with a defect in cytokinesis

Larry H. Thompson, Patricia A. Lindl

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

In a selection procedure designed to enrich for temperature-sensitive mutant cells blocked in mitosis a CHO-cell mutant was isolated which has a defect in cytokinesis as the basis of its temperature-sensitive phenotype. Cultures of the mutant had an abnormally high percentage (ie, 34%) of polyploid cells at the permissive temperature of 34°C and showed further increased frequencies of polyploidy as well as many multinucleated cells at 38.5° and 39.5°. When the mutant cells were synchronized in metaphase by Colcemid arrest and then placed into fresh medium at nonpermissive temperature, they did not divide although the completion of mitosis appeared cytologically normal. Ultrastructural examination by electron microscopy of such synchronized cells at telophase revealed no specific defects in cellular components other than failure of development of a normal midbody. The sensitivity of the mutant to cytochalasin B and to Colcemid was the same as for wild-type cells. This mutation behaved as recessive in tetraploid cell hybrids constructed by fusing the mutant with a CHO strain which was wild-type with respect to temperature sensitivity.

Original languageEnglish (US)
Pages (from-to)387-400
Number of pages14
JournalSomatic Cell Genetics
Volume2
Issue number5
DOIs
StatePublished - Sep 1976
Externally publishedYes

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Cytokinesis
CHO Cells
Temperature
Demecolcine
Polyploidy
Mitosis
Telophase
Cytochalasin B
Tetraploidy
Hybrid Cells
Metaphase
Electron Microscopy
Phenotype
Mutation

ASJC Scopus subject areas

  • Genetics
  • Medicine(all)

Cite this

A CHO-cell mutant with a defect in cytokinesis. / Thompson, Larry H.; Lindl, Patricia A.

In: Somatic Cell Genetics, Vol. 2, No. 5, 09.1976, p. 387-400.

Research output: Contribution to journalArticle

Thompson, Larry H. ; Lindl, Patricia A. / A CHO-cell mutant with a defect in cytokinesis. In: Somatic Cell Genetics. 1976 ; Vol. 2, No. 5. pp. 387-400.
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