A Chinese hamster ovary cell mutant (EM-C11) with sensitivity to simple alkylating agents and a very high level of sister chromatid exchanges

M. Z. Zdzienicka, G. P. Van Der Schans, A. T. Natarajan, L. H. Thompson, I. Neuteboom, J. W I M Simons

Research output: Contribution to journalArticle

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Abstract

We have isolated a Chinese hamster ovary cell mutant hypersensitive to monofunctional alkylating agents. The mutant, designed as EM-C11, showed hypersensitivity to ethyl methanesulfonate (EMS), methyl methanesulfonate and ethylnitrosourea (8-, 7- and 2-fold, respectively, based on D10 values). About 2-fold increased sensitivity towards 4-nitroquinoline-1-oxide and only slightly increased sensitivity to X-rays (1.4-fold) and mitomycin C treatment (1.6-fold) were found in this mutant. EM-C11 was not hypersensitive to UV irradiation nor to adriamycin. The EM-C11 cells showed ∼10-fold higher level of spontaneous sister chromatid exchange. The level of spontaneous chromosomal aberrations was 2- to 3-fold higher, but the frequency of EMS-induced chromosomal aberrations was ∼10-fold higher in the mutant cells, in agreement with the increased sensitivity to killing. As measured by alkaline elution, EM-C11 cells showed a defect in the rejoining of single-strand DNA breaks after exposure to X-rays and even more so after the EMS treatment. Genetic analysis revealed that the EM-C11 mutant belongs to the same complementation group as the EM9 mutant described earlier. The XRCC1 gene which complements the defect in EM9 also complements the defect in EM-C11, confirming that these two independently isolated mutants are defective in the same gene.

Original languageEnglish (US)
Pages (from-to)265-269
Number of pages5
JournalMutagenesis
Volume7
Issue number4
DOIs
StatePublished - Jul 1992
Externally publishedYes

Fingerprint

Ethyl Methanesulfonate
Ovary
Sister Chromatid Exchange
Alkylating Agents
Cricetulus
Mutant
Fold
Cells
Aberrations
Defects
Cell
Genes
Chromosome Aberrations
4-Nitroquinoline-1-oxide
Ethylnitrosourea
Methyl Methanesulfonate
X rays
X-Rays
Mitomycin
Single-Stranded DNA Breaks

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Zdzienicka, M. Z., Van Der Schans, G. P., Natarajan, A. T., Thompson, L. H., Neuteboom, I., & Simons, J. W. I. M. (1992). A Chinese hamster ovary cell mutant (EM-C11) with sensitivity to simple alkylating agents and a very high level of sister chromatid exchanges. Mutagenesis, 7(4), 265-269. https://doi.org/10.1093/mutage/7.4.265

A Chinese hamster ovary cell mutant (EM-C11) with sensitivity to simple alkylating agents and a very high level of sister chromatid exchanges. / Zdzienicka, M. Z.; Van Der Schans, G. P.; Natarajan, A. T.; Thompson, L. H.; Neuteboom, I.; Simons, J. W I M.

In: Mutagenesis, Vol. 7, No. 4, 07.1992, p. 265-269.

Research output: Contribution to journalArticle

Zdzienicka, MZ, Van Der Schans, GP, Natarajan, AT, Thompson, LH, Neuteboom, I & Simons, JWIM 1992, 'A Chinese hamster ovary cell mutant (EM-C11) with sensitivity to simple alkylating agents and a very high level of sister chromatid exchanges', Mutagenesis, vol. 7, no. 4, pp. 265-269. https://doi.org/10.1093/mutage/7.4.265
Zdzienicka, M. Z. ; Van Der Schans, G. P. ; Natarajan, A. T. ; Thompson, L. H. ; Neuteboom, I. ; Simons, J. W I M. / A Chinese hamster ovary cell mutant (EM-C11) with sensitivity to simple alkylating agents and a very high level of sister chromatid exchanges. In: Mutagenesis. 1992 ; Vol. 7, No. 4. pp. 265-269.
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