A CD8/Lck transgene is able to drive thymocyte differentiation

Ruben C Fragoso, Saiju Pyarajan, Hanna Yoko Irie, Steven J. Burakoff

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Efficient development of thymocytes requires participation of a CD8 or CD4 coreceptor in the TCR:MHC interaction. Both CD8 and CD4 coreceptor cytoplasmic domains associate with Lck. In this study, we attempted to delineate the role of CD8α-associated Lck in driving CDS single positive (SP) thymocyte development We used a chimeric molecule encoding the extracellular and transmembrane domains of CD8α fused to full-length Lck. In mice deficient for CD8α and transgenic for 2C, a MHC class I-restricted TCR, robust reconstitution of CD8 SP thymocytes occurred both centrally and peripherally. The reconstituted CD8 SP population was phenotypically and functionally comparable to 2C wild-type counterparts expressing endogenous CD8α. A CD8α/Lck kinase-dead chimera also resulted in reconstitution of CDS SP thymocytes. Our results suggest that CD8α-associated Lck is sufficient to drive CD8 SP thymocyte development. Furthermore, this CDS SP development may not necessarily depend on Lck kinase activity.

Original languageEnglish (US)
Pages (from-to)6007-6017
Number of pages11
JournalJournal of Immunology
Volume177
Issue number9
StatePublished - Nov 1 2006
Externally publishedYes

ASJC Scopus subject areas

  • Immunology

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    Fragoso, R. C., Pyarajan, S., Irie, H. Y., & Burakoff, S. J. (2006). A CD8/Lck transgene is able to drive thymocyte differentiation. Journal of Immunology, 177(9), 6007-6017.