A bovine model of vaccine enhanced respiratory syncytial virus pathophysiology

Laurel J Gershwin, Edward S Schelegle, Robert A. Gunther, Mark L Anderson, Amelia R. Woolums, Danielle R. Larochelle, Gabrielle A. Boyle, Kathleen E. Friebertshauser, Randall S. Singer

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

A critical issue has been the observation that vaccination of children with a formalin-inactivated respiratory syncytial virus (RSV) vaccine is associated with disease enhancement. We have taken advantage of bovine RSV and our experience with this disease in calves to develop a natural model that parallels human disease. Using formalin-inactivated bovine RSV vaccine calves were either sham-vaccinated/infected, vaccinated/infected, or vaccinated/sham-infected and their clinical signs, pulmonary function, and histological lung lesions quantitatively scored. Interestingly there was significantly greater disease in vaccinated/infected calves and histological lesions in calves were similar to those of affected children. Finally, we note that vaccination did not induce neutralizing antibodies, but IgG antibodies were detected by ELISA. Our model of RSV enhanced disease is important because it provides quantifiable evidence of disease severity that can be applied to evaluate the mechanisms of immunopathology and the safety of candidate RSV vaccines.

Original languageEnglish (US)
Pages (from-to)1225-1236
Number of pages12
JournalVaccine
Volume16
Issue number11-12
DOIs
StatePublished - 1998

Fingerprint

Respiratory Syncytial Virus Vaccines
pathophysiology
calves
vaccines
Bovine respiratory syncytial virus
viruses
cattle
formalin
lesions (animal)
vaccination
Formaldehyde
immunopathology
Vaccination
lung function
Lung
neutralizing antibodies
human diseases
Inactivated Vaccines
Respiratory Syncytial Viruses
disease severity

Keywords

  • Bovine respiratory syncytial virus
  • Vaccine

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

A bovine model of vaccine enhanced respiratory syncytial virus pathophysiology. / Gershwin, Laurel J; Schelegle, Edward S; Gunther, Robert A.; Anderson, Mark L; Woolums, Amelia R.; Larochelle, Danielle R.; Boyle, Gabrielle A.; Friebertshauser, Kathleen E.; Singer, Randall S.

In: Vaccine, Vol. 16, No. 11-12, 1998, p. 1225-1236.

Research output: Contribution to journalArticle

Gershwin, LJ, Schelegle, ES, Gunther, RA, Anderson, ML, Woolums, AR, Larochelle, DR, Boyle, GA, Friebertshauser, KE & Singer, RS 1998, 'A bovine model of vaccine enhanced respiratory syncytial virus pathophysiology', Vaccine, vol. 16, no. 11-12, pp. 1225-1236. https://doi.org/10.1016/S0264-410X(98)80123-0
Gershwin, Laurel J ; Schelegle, Edward S ; Gunther, Robert A. ; Anderson, Mark L ; Woolums, Amelia R. ; Larochelle, Danielle R. ; Boyle, Gabrielle A. ; Friebertshauser, Kathleen E. ; Singer, Randall S. / A bovine model of vaccine enhanced respiratory syncytial virus pathophysiology. In: Vaccine. 1998 ; Vol. 16, No. 11-12. pp. 1225-1236.
@article{7b912806eed84390bbe36b75061c71e0,
title = "A bovine model of vaccine enhanced respiratory syncytial virus pathophysiology",
abstract = "A critical issue has been the observation that vaccination of children with a formalin-inactivated respiratory syncytial virus (RSV) vaccine is associated with disease enhancement. We have taken advantage of bovine RSV and our experience with this disease in calves to develop a natural model that parallels human disease. Using formalin-inactivated bovine RSV vaccine calves were either sham-vaccinated/infected, vaccinated/infected, or vaccinated/sham-infected and their clinical signs, pulmonary function, and histological lung lesions quantitatively scored. Interestingly there was significantly greater disease in vaccinated/infected calves and histological lesions in calves were similar to those of affected children. Finally, we note that vaccination did not induce neutralizing antibodies, but IgG antibodies were detected by ELISA. Our model of RSV enhanced disease is important because it provides quantifiable evidence of disease severity that can be applied to evaluate the mechanisms of immunopathology and the safety of candidate RSV vaccines.",
keywords = "Bovine respiratory syncytial virus, Vaccine",
author = "Gershwin, {Laurel J} and Schelegle, {Edward S} and Gunther, {Robert A.} and Anderson, {Mark L} and Woolums, {Amelia R.} and Larochelle, {Danielle R.} and Boyle, {Gabrielle A.} and Friebertshauser, {Kathleen E.} and Singer, {Randall S.}",
year = "1998",
doi = "10.1016/S0264-410X(98)80123-0",
language = "English (US)",
volume = "16",
pages = "1225--1236",
journal = "Vaccine",
issn = "0264-410X",
publisher = "Elsevier BV",
number = "11-12",

}

TY - JOUR

T1 - A bovine model of vaccine enhanced respiratory syncytial virus pathophysiology

AU - Gershwin, Laurel J

AU - Schelegle, Edward S

AU - Gunther, Robert A.

AU - Anderson, Mark L

AU - Woolums, Amelia R.

AU - Larochelle, Danielle R.

AU - Boyle, Gabrielle A.

AU - Friebertshauser, Kathleen E.

AU - Singer, Randall S.

PY - 1998

Y1 - 1998

N2 - A critical issue has been the observation that vaccination of children with a formalin-inactivated respiratory syncytial virus (RSV) vaccine is associated with disease enhancement. We have taken advantage of bovine RSV and our experience with this disease in calves to develop a natural model that parallels human disease. Using formalin-inactivated bovine RSV vaccine calves were either sham-vaccinated/infected, vaccinated/infected, or vaccinated/sham-infected and their clinical signs, pulmonary function, and histological lung lesions quantitatively scored. Interestingly there was significantly greater disease in vaccinated/infected calves and histological lesions in calves were similar to those of affected children. Finally, we note that vaccination did not induce neutralizing antibodies, but IgG antibodies were detected by ELISA. Our model of RSV enhanced disease is important because it provides quantifiable evidence of disease severity that can be applied to evaluate the mechanisms of immunopathology and the safety of candidate RSV vaccines.

AB - A critical issue has been the observation that vaccination of children with a formalin-inactivated respiratory syncytial virus (RSV) vaccine is associated with disease enhancement. We have taken advantage of bovine RSV and our experience with this disease in calves to develop a natural model that parallels human disease. Using formalin-inactivated bovine RSV vaccine calves were either sham-vaccinated/infected, vaccinated/infected, or vaccinated/sham-infected and their clinical signs, pulmonary function, and histological lung lesions quantitatively scored. Interestingly there was significantly greater disease in vaccinated/infected calves and histological lesions in calves were similar to those of affected children. Finally, we note that vaccination did not induce neutralizing antibodies, but IgG antibodies were detected by ELISA. Our model of RSV enhanced disease is important because it provides quantifiable evidence of disease severity that can be applied to evaluate the mechanisms of immunopathology and the safety of candidate RSV vaccines.

KW - Bovine respiratory syncytial virus

KW - Vaccine

UR - http://www.scopus.com/inward/record.url?scp=0031779850&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0031779850&partnerID=8YFLogxK

U2 - 10.1016/S0264-410X(98)80123-0

DO - 10.1016/S0264-410X(98)80123-0

M3 - Article

C2 - 9682383

AN - SCOPUS:0031779850

VL - 16

SP - 1225

EP - 1236

JO - Vaccine

JF - Vaccine

SN - 0264-410X

IS - 11-12

ER -