A blocking ELISA for detection of antibody to a subgroup-reactive epitope of African horsesickness viral protein 7 (VP7) using a novel gamma-irradiated antigen

J. A. House; Jeffrey L Stott; Myra Blanchard; M. LaRocco; M. E. Llewellyn

A blocking ELISA for detection of antibody to a subgroup-reactive epitope of African horsesickness viral protein 7 (VP7) using a novel gamma-irradiated antigen

J. A. House, Jeffrey L Stott, Myra Blanchard, M. LaRocco, M. E. Llewellyn

Research output: Contribution to journal › Article › peer-review

Abstract

A novel gamma irradiated inactivated cell culture derived African horsesickness viral (AHSV) antigen was used in a blocking ELISA (B-ELISA) for detecting antibody to a subgroup-reactive epitope of AHSV. A monoclonal antibody (MAB), class IgM, against an epitope on African horsesickness (AHS) viral protein 7 (VP7) was developed in BALBc mice and used in the B-ELISA. The MAB, designated F9H, was blocked by 69 serums from equidae with antibody to AHS, but its binding activity was not appreciably affected by 301 serums that did not contain antibodies to AHS virus. An ELISA protocol using a blocking format is described.

Original language	English (US)
Pages (from-to)	333-344
Number of pages	12
Journal	Annals of the New York Academy of Sciences
Volume	791
State	Published - 1996

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

Cite this

A blocking ELISA for detection of antibody to a subgroup-reactive epitope of African horsesickness viral protein 7 (VP7) using a novel gamma-irradiated antigen. / House, J. A.; Stott, Jeffrey L ; Blanchard, Myra; LaRocco, M.; Llewellyn, M. E.

In: Annals of the New York Academy of Sciences, Vol. 791, 1996, p. 333-344.

Research output: Contribution to journal › Article › peer-review

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AU - LaRocco, M.

AU - Llewellyn, M. E.

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AB - A novel gamma irradiated inactivated cell culture derived African horsesickness viral (AHSV) antigen was used in a blocking ELISA (B-ELISA) for detecting antibody to a subgroup-reactive epitope of AHSV. A monoclonal antibody (MAB), class IgM, against an epitope on African horsesickness (AHS) viral protein 7 (VP7) was developed in BALBc mice and used in the B-ELISA. The MAB, designated F9H, was blocked by 69 serums from equidae with antibody to AHS, but its binding activity was not appreciably affected by 301 serums that did not contain antibodies to AHS virus. An ELISA protocol using a blocking format is described.

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A blocking ELISA for detection of antibody to a subgroup-reactive epitope of African horsesickness viral protein 7 (VP7) using a novel gamma-irradiated antigen

Abstract

ASJC Scopus subject areas

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