A blocking ELISA for detection of antibody to a subgroup-reactive epitope of African horsesickness viral protein 7 (VP7) using a novel gamma-irradiated antigen

J. A. House, Jeffrey L Stott, Myra Blanchard, M. LaRocco, M. E. Llewellyn

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A novel gamma irradiated inactivated cell culture derived African horsesickness viral (AHSV) antigen was used in a blocking ELISA (B-ELISA) for detecting antibody to a subgroup-reactive epitope of AHSV. A monoclonal antibody (MAB), class IgM, against an epitope on African horsesickness (AHS) viral protein 7 (VP7) was developed in BALBc mice and used in the B-ELISA. The MAB, designated F9H, was blocked by 69 serums from equidae with antibody to AHS, but its binding activity was not appreciably affected by 301 serums that did not contain antibodies to AHS virus. An ELISA protocol using a blocking format is described.

Original languageEnglish (US)
Pages (from-to)333-344
Number of pages12
JournalAnnals of the New York Academy of Sciences
Volume791
StatePublished - 1996

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African Horse Sickness
Viral Proteins
Epitopes
Enzyme-Linked Immunosorbent Assay
Antigens
Antibodies
Monoclonal Antibodies
Viral Antigens
Immunoglobulin Isotypes
Viruses
Cell culture
Immunoglobulin M
Equidae
Serum
Cell Culture Techniques
Protein
Africa

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

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abstract = "A novel gamma irradiated inactivated cell culture derived African horsesickness viral (AHSV) antigen was used in a blocking ELISA (B-ELISA) for detecting antibody to a subgroup-reactive epitope of AHSV. A monoclonal antibody (MAB), class IgM, against an epitope on African horsesickness (AHS) viral protein 7 (VP7) was developed in BALBc mice and used in the B-ELISA. The MAB, designated F9H, was blocked by 69 serums from equidae with antibody to AHS, but its binding activity was not appreciably affected by 301 serums that did not contain antibodies to AHS virus. An ELISA protocol using a blocking format is described.",
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T1 - A blocking ELISA for detection of antibody to a subgroup-reactive epitope of African horsesickness viral protein 7 (VP7) using a novel gamma-irradiated antigen

AU - House, J. A.

AU - Stott, Jeffrey L

AU - Blanchard, Myra

AU - LaRocco, M.

AU - Llewellyn, M. E.

PY - 1996

Y1 - 1996

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