Abstract
A novel gamma irradiated inactivated cell culture derived African horsesickness viral (AHSV) antigen was used in a blocking ELISA (B-ELISA) for detecting antibody to a subgroup-reactive epitope of AHSV. A monoclonal antibody (MAB), class IgM, against an epitope on African horsesickness (AHS) viral protein 7 (VP7) was developed in BALBc mice and used in the B-ELISA. The MAB, designated F9H, was blocked by 69 serums from equidae with antibody to AHS, but its binding activity was not appreciably affected by 301 serums that did not contain antibodies to AHS virus. An ELISA protocol using a blocking format is described.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 333-344 |
| Number of pages | 12 |
| Journal | Annals of the New York Academy of Sciences |
| Volume | 791 |
| State | Published - 1996 |
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ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
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A blocking ELISA for detection of antibody to a subgroup-reactive epitope of African horsesickness viral protein 7 (VP7) using a novel gamma-irradiated antigen. / House, J. A.; Stott, Jeffrey L; Blanchard, Myra; LaRocco, M.; Llewellyn, M. E.
In: Annals of the New York Academy of Sciences, Vol. 791, 1996, p. 333-344.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A blocking ELISA for detection of antibody to a subgroup-reactive epitope of African horsesickness viral protein 7 (VP7) using a novel gamma-irradiated antigen
AU - House, J. A.
AU - Stott, Jeffrey L
AU - Blanchard, Myra
AU - LaRocco, M.
AU - Llewellyn, M. E.
PY - 1996
Y1 - 1996
N2 - A novel gamma irradiated inactivated cell culture derived African horsesickness viral (AHSV) antigen was used in a blocking ELISA (B-ELISA) for detecting antibody to a subgroup-reactive epitope of AHSV. A monoclonal antibody (MAB), class IgM, against an epitope on African horsesickness (AHS) viral protein 7 (VP7) was developed in BALBc mice and used in the B-ELISA. The MAB, designated F9H, was blocked by 69 serums from equidae with antibody to AHS, but its binding activity was not appreciably affected by 301 serums that did not contain antibodies to AHS virus. An ELISA protocol using a blocking format is described.
AB - A novel gamma irradiated inactivated cell culture derived African horsesickness viral (AHSV) antigen was used in a blocking ELISA (B-ELISA) for detecting antibody to a subgroup-reactive epitope of AHSV. A monoclonal antibody (MAB), class IgM, against an epitope on African horsesickness (AHS) viral protein 7 (VP7) was developed in BALBc mice and used in the B-ELISA. The MAB, designated F9H, was blocked by 69 serums from equidae with antibody to AHS, but its binding activity was not appreciably affected by 301 serums that did not contain antibodies to AHS virus. An ELISA protocol using a blocking format is described.
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UR - http://www.scopus.com/inward/citedby.url?scp=0029760286&partnerID=8YFLogxK
M3 - Article
C2 - 8784514
AN - SCOPUS:0029760286
VL - 791
SP - 333
EP - 344
JO - Annals of the New York Academy of Sciences
JF - Annals of the New York Academy of Sciences
SN - 0077-8923
ER -