A bistable hysteretic switch in an activator-repressor regulated restriction-modification system

Kristen Williams, Michael A. Savageau, Robert M. Blumenthal

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Restriction-modification (RM) systems are extremely widespread among bacteria and archaea, and are often specified by mobile genetic elements. In type II RM systems, where the restriction endonuclease (REase) and protective DNA methyltransferase (MTase) are separate proteins, a major regulatory challenge is delaying expression of the REase relative to the MTase after RM genes enter a new host cell. Basic understanding of this regulation is available for few RM systems, and detailed understanding for none. The PvuII RM system is one of a large subset in which the central regulatory role is played by an activator-repressor protein (called C, for controller). REase expression depends upon activation by C, whereas expression of the MTase does not. Thus delay of REase expression depends on the rate of C-protein accumulation. This is a nonlinear process, as C also activates transcription of its own gene. Mathematical modeling of the PvuII system led to the unexpected predictions of responsiveness to a factor not previously studied in RM system control - gene copy number - and of a hysteretic response. In this study, those predictions have been confirmed experimentally. The results may apply to many other C-regulated RM systems, and help explain their ability to spread so widely.

Original languageEnglish (US)
Pages (from-to)6045-6057
Number of pages13
JournalNucleic Acids Research
Volume41
Issue number12
DOIs
StatePublished - Jul 2013

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DNA Restriction-Modification Enzymes
DNA Restriction Enzymes
Methyltransferases
Protein C
Protein Methyltransferases
Interspersed Repetitive Sequences
Repressor Proteins
Gene Dosage
Archaea
Genes
Bacteria
DNA

ASJC Scopus subject areas

  • Genetics

Cite this

A bistable hysteretic switch in an activator-repressor regulated restriction-modification system. / Williams, Kristen; Savageau, Michael A.; Blumenthal, Robert M.

In: Nucleic Acids Research, Vol. 41, No. 12, 07.2013, p. 6045-6057.

Research output: Contribution to journalArticle

Williams, Kristen ; Savageau, Michael A. ; Blumenthal, Robert M. / A bistable hysteretic switch in an activator-repressor regulated restriction-modification system. In: Nucleic Acids Research. 2013 ; Vol. 41, No. 12. pp. 6045-6057.
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