A bipolar kinesin

A. S. Kashina, R. J. Baskin, D. G. Cole, K. P. Wedaman, W. M. Saxton, J. M. Scholey

Research output: Contribution to journalArticle

262 Citations (Scopus)

Abstract

CHROMOSOME segregation during mitosis depends on the action of the mitotic spindle, a self organizing, bipolar protein machine which uses microtubules (MTs) and their associated motors. Members of the BimC subfamily of kinesin related MT-motor proteins are believed to be essential for the formation and functioning of a normal bipolar spindle. Here we report that KRP130, a homotetrameric BimC-related kinesin purified from Drosophila melanogaster embryos, has an unusual ultrastructure. It consists of four kinesin-related polypeptides assembled into a bipolar aggregate with motor domains at opposite ends, analogous to a miniature myosin filament. Such a bipolar 'minifilament' could crosslink spindle MTs and slide them relative to one another. We do not know of any other MT motors that have a bipolar structure.

Original languageEnglish (US)
Pages (from-to)270-272
Number of pages3
JournalNature
Volume379
Issue number6562
DOIs
StatePublished - Jan 18 1996

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Kinesin
Microtubules
Microtubule Proteins
Spindle Apparatus
Myosins
Drosophila melanogaster
Mitosis
Embryonic Structures
Peptides
Proteins

ASJC Scopus subject areas

  • General

Cite this

Kashina, A. S., Baskin, R. J., Cole, D. G., Wedaman, K. P., Saxton, W. M., & Scholey, J. M. (1996). A bipolar kinesin. Nature, 379(6562), 270-272. https://doi.org/10.1038/379270a0

A bipolar kinesin. / Kashina, A. S.; Baskin, R. J.; Cole, D. G.; Wedaman, K. P.; Saxton, W. M.; Scholey, J. M.

In: Nature, Vol. 379, No. 6562, 18.01.1996, p. 270-272.

Research output: Contribution to journalArticle

Kashina, AS, Baskin, RJ, Cole, DG, Wedaman, KP, Saxton, WM & Scholey, JM 1996, 'A bipolar kinesin', Nature, vol. 379, no. 6562, pp. 270-272. https://doi.org/10.1038/379270a0
Kashina AS, Baskin RJ, Cole DG, Wedaman KP, Saxton WM, Scholey JM. A bipolar kinesin. Nature. 1996 Jan 18;379(6562):270-272. https://doi.org/10.1038/379270a0
Kashina, A. S. ; Baskin, R. J. ; Cole, D. G. ; Wedaman, K. P. ; Saxton, W. M. ; Scholey, J. M. / A bipolar kinesin. In: Nature. 1996 ; Vol. 379, No. 6562. pp. 270-272.
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