A bipolar kinesin

A. S. Kashina; R. J. Baskin; D. G. Cole; K. P. Wedaman; W. M. Saxton; J. M. Scholey

doi:10.1038/379270a0

A bipolar kinesin

A. S. Kashina, R. J. Baskin, D. G. Cole, K. P. Wedaman, W. M. Saxton, J. M. Scholey

Molecular and Cellular Biology

Research output: Contribution to journal › Article

262 Citations (Scopus)

Abstract

CHROMOSOME segregation during mitosis depends on the action of the mitotic spindle, a self organizing, bipolar protein machine which uses microtubules (MTs) and their associated motors. Members of the BimC subfamily of kinesin related MT-motor proteins are believed to be essential for the formation and functioning of a normal bipolar spindle. Here we report that KRP₁₃₀, a homotetrameric BimC-related kinesin purified from Drosophila melanogaster embryos, has an unusual ultrastructure. It consists of four kinesin-related polypeptides assembled into a bipolar aggregate with motor domains at opposite ends, analogous to a miniature myosin filament. Such a bipolar 'minifilament' could crosslink spindle MTs and slide them relative to one another. We do not know of any other MT motors that have a bipolar structure.

Original language	English (US)
Pages (from-to)	270-272
Number of pages	3
Journal	Nature
Volume	379
Issue number	6562
DOIs	https://doi.org/10.1038/379270a0
State	Published - Jan 18 1996

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Kinesin

Microtubules

Microtubule Proteins

Spindle Apparatus

Myosins

Drosophila melanogaster

Mitosis

Embryonic Structures

Peptides

Proteins

ASJC Scopus subject areas

General

Cite this

Kashina, A. S., Baskin, R. J., Cole, D. G., Wedaman, K. P., Saxton, W. M., & Scholey, J. M. (1996). A bipolar kinesin. Nature, 379(6562), 270-272. https://doi.org/10.1038/379270a0

A bipolar kinesin. / Kashina, A. S.; Baskin, R. J.; Cole, D. G.; Wedaman, K. P.; Saxton, W. M.; Scholey, J. M.

In: Nature, Vol. 379, No. 6562, 18.01.1996, p. 270-272.

Research output: Contribution to journal › Article

Kashina, AS, Baskin, RJ, Cole, DG, Wedaman, KP, Saxton, WM & Scholey, JM 1996, 'A bipolar kinesin', Nature, vol. 379, no. 6562, pp. 270-272. https://doi.org/10.1038/379270a0

Kashina AS, Baskin RJ, Cole DG, Wedaman KP, Saxton WM, Scholey JM. A bipolar kinesin. Nature. 1996 Jan 18;379(6562):270-272. https://doi.org/10.1038/379270a0

Kashina, A. S. ; Baskin, R. J. ; Cole, D. G. ; Wedaman, K. P. ; Saxton, W. M. ; Scholey, J. M. / A bipolar kinesin. In: Nature. 1996 ; Vol. 379, No. 6562. pp. 270-272.

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