A bipolar kinesin

A. S. Kashina; R. J. Baskin; D. G. Cole; K. P. Wedaman; W. M. Saxton; J. M. Scholey

doi:10.1038/379270a0

A bipolar kinesin

A. S. Kashina, R. J. Baskin, D. G. Cole, K. P. Wedaman, W. M. Saxton, J. M. Scholey

Molecular and Cellular Biology

Research output: Contribution to journal › Article › peer-review

273 Scopus citations

Abstract

CHROMOSOME segregation during mitosis depends on the action of the mitotic spindle, a self organizing, bipolar protein machine which uses microtubules (MTs) and their associated motors. Members of the BimC subfamily of kinesin related MT-motor proteins are believed to be essential for the formation and functioning of a normal bipolar spindle. Here we report that KRP₁₃₀, a homotetrameric BimC-related kinesin purified from Drosophila melanogaster embryos, has an unusual ultrastructure. It consists of four kinesin-related polypeptides assembled into a bipolar aggregate with motor domains at opposite ends, analogous to a miniature myosin filament. Such a bipolar 'minifilament' could crosslink spindle MTs and slide them relative to one another. We do not know of any other MT motors that have a bipolar structure.

Original language	English (US)
Pages (from-to)	270-272
Number of pages	3
Journal	Nature
Volume	379
Issue number	6562
DOIs	https://doi.org/10.1038/379270a0
State	Published - Jan 18 1996

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title = "A bipolar kinesin",

abstract = "CHROMOSOME segregation during mitosis depends on the action of the mitotic spindle, a self organizing, bipolar protein machine which uses microtubules (MTs) and their associated motors. Members of the BimC subfamily of kinesin related MT-motor proteins are believed to be essential for the formation and functioning of a normal bipolar spindle. Here we report that KRP130, a homotetrameric BimC-related kinesin purified from Drosophila melanogaster embryos, has an unusual ultrastructure. It consists of four kinesin-related polypeptides assembled into a bipolar aggregate with motor domains at opposite ends, analogous to a miniature myosin filament. Such a bipolar 'minifilament' could crosslink spindle MTs and slide them relative to one another. We do not know of any other MT motors that have a bipolar structure.",

author = "Kashina, {A. S.} and Baskin, {R. J.} and Cole, {D. G.} and Wedaman, {K. P.} and Saxton, {W. M.} and Scholey, {J. M.}",

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T1 - A bipolar kinesin

AU - Kashina, A. S.

AU - Baskin, R. J.

AU - Cole, D. G.

AU - Wedaman, K. P.

AU - Saxton, W. M.

AU - Scholey, J. M.

PY - 1996/1/18

Y1 - 1996/1/18

N2 - CHROMOSOME segregation during mitosis depends on the action of the mitotic spindle, a self organizing, bipolar protein machine which uses microtubules (MTs) and their associated motors. Members of the BimC subfamily of kinesin related MT-motor proteins are believed to be essential for the formation and functioning of a normal bipolar spindle. Here we report that KRP130, a homotetrameric BimC-related kinesin purified from Drosophila melanogaster embryos, has an unusual ultrastructure. It consists of four kinesin-related polypeptides assembled into a bipolar aggregate with motor domains at opposite ends, analogous to a miniature myosin filament. Such a bipolar 'minifilament' could crosslink spindle MTs and slide them relative to one another. We do not know of any other MT motors that have a bipolar structure.

AB - CHROMOSOME segregation during mitosis depends on the action of the mitotic spindle, a self organizing, bipolar protein machine which uses microtubules (MTs) and their associated motors. Members of the BimC subfamily of kinesin related MT-motor proteins are believed to be essential for the formation and functioning of a normal bipolar spindle. Here we report that KRP130, a homotetrameric BimC-related kinesin purified from Drosophila melanogaster embryos, has an unusual ultrastructure. It consists of four kinesin-related polypeptides assembled into a bipolar aggregate with motor domains at opposite ends, analogous to a miniature myosin filament. Such a bipolar 'minifilament' could crosslink spindle MTs and slide them relative to one another. We do not know of any other MT motors that have a bipolar structure.

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A bipolar kinesin

Abstract

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