Abstract
Current biomaterials for auricular replacement are associated with high rates of infection and extrusion. The development of new auricular biomaterials that mimic the mechanical properties of native tissue and promote desirable cellular interactions may prevent implant failure. A porous 3D nanocomposite scaffold (NS) based on POSS-PCU (polyhedral oligomeric silsesquioxane nanocage into polycarbonate based urea-urethane) is developed with an elastic modulus similar to native ear. In vitro biological interactions on this NS reveal greater protein adsorption, increased fibroblast adhesion, proliferation, and collagen production compared with Medpor (the current synthetic auricular implant). In vivo, the POSS-PCU with larger pores (NS2; 150-250 μm) have greater tissue ingrowth (≈5.8× and ≈1.4 × increase) than the POSS-PCU with smaller pores (NS1; 100-50 μm) and when compared to Medpor (>100 μm). The NS2 with the larger pores demonstrates a reduced fibrotic encapsulation compared with NS1 and Medpor (≈4.1× and ≈1.6×, respectively; P < 0.05). Porosity also influences the amount of neovascularization within the implants, with no blood vessel observed in NS1 (12 weeks postimplantation). The lack of chronic inflammatory response for all materials may indicate that the elastic modulus and pore size of the implant scaffold could be important design considerations for influencing fibrotic responses to auricular and other soft tissue implants.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1203-1212 |
| Number of pages | 10 |
| Journal | Advanced healthcare materials |
| Volume | 5 |
| Issue number | 10 |
| DOIs | |
| State | Published - May 25 2016 |
| Externally published | Yes |
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Keywords
- Auricular cartilage
- Biomaterial
- Medpor
- Nanocomposite scaffold
- POSS-PCU
ASJC Scopus subject areas
- Biomedical Engineering
- Biomaterials
- Pharmaceutical Science
Cite this
A Biodesigned Nanocomposite Biomaterial for Auricular Cartilage Reconstruction. / Nayyer, Leila; Jell, Gavin; Esmaeili, Ali; Birchall, Martin; Seifalian, Alexander M.
In: Advanced healthcare materials, Vol. 5, No. 10, 25.05.2016, p. 1203-1212.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - A Biodesigned Nanocomposite Biomaterial for Auricular Cartilage Reconstruction
AU - Nayyer, Leila
AU - Jell, Gavin
AU - Esmaeili, Ali
AU - Birchall, Martin
AU - Seifalian, Alexander M.
PY - 2016/5/25
Y1 - 2016/5/25
N2 - Current biomaterials for auricular replacement are associated with high rates of infection and extrusion. The development of new auricular biomaterials that mimic the mechanical properties of native tissue and promote desirable cellular interactions may prevent implant failure. A porous 3D nanocomposite scaffold (NS) based on POSS-PCU (polyhedral oligomeric silsesquioxane nanocage into polycarbonate based urea-urethane) is developed with an elastic modulus similar to native ear. In vitro biological interactions on this NS reveal greater protein adsorption, increased fibroblast adhesion, proliferation, and collagen production compared with Medpor (the current synthetic auricular implant). In vivo, the POSS-PCU with larger pores (NS2; 150-250 μm) have greater tissue ingrowth (≈5.8× and ≈1.4 × increase) than the POSS-PCU with smaller pores (NS1; 100-50 μm) and when compared to Medpor (>100 μm). The NS2 with the larger pores demonstrates a reduced fibrotic encapsulation compared with NS1 and Medpor (≈4.1× and ≈1.6×, respectively; P < 0.05). Porosity also influences the amount of neovascularization within the implants, with no blood vessel observed in NS1 (12 weeks postimplantation). The lack of chronic inflammatory response for all materials may indicate that the elastic modulus and pore size of the implant scaffold could be important design considerations for influencing fibrotic responses to auricular and other soft tissue implants.
AB - Current biomaterials for auricular replacement are associated with high rates of infection and extrusion. The development of new auricular biomaterials that mimic the mechanical properties of native tissue and promote desirable cellular interactions may prevent implant failure. A porous 3D nanocomposite scaffold (NS) based on POSS-PCU (polyhedral oligomeric silsesquioxane nanocage into polycarbonate based urea-urethane) is developed with an elastic modulus similar to native ear. In vitro biological interactions on this NS reveal greater protein adsorption, increased fibroblast adhesion, proliferation, and collagen production compared with Medpor (the current synthetic auricular implant). In vivo, the POSS-PCU with larger pores (NS2; 150-250 μm) have greater tissue ingrowth (≈5.8× and ≈1.4 × increase) than the POSS-PCU with smaller pores (NS1; 100-50 μm) and when compared to Medpor (>100 μm). The NS2 with the larger pores demonstrates a reduced fibrotic encapsulation compared with NS1 and Medpor (≈4.1× and ≈1.6×, respectively; P < 0.05). Porosity also influences the amount of neovascularization within the implants, with no blood vessel observed in NS1 (12 weeks postimplantation). The lack of chronic inflammatory response for all materials may indicate that the elastic modulus and pore size of the implant scaffold could be important design considerations for influencing fibrotic responses to auricular and other soft tissue implants.
KW - Auricular cartilage
KW - Biomaterial
KW - Medpor
KW - Nanocomposite scaffold
KW - POSS-PCU
UR - http://www.scopus.com/inward/record.url?scp=84961226367&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84961226367&partnerID=8YFLogxK
U2 - 10.1002/adhm.201500968
DO - 10.1002/adhm.201500968
M3 - Article
C2 - 26992039
AN - SCOPUS:84961226367
VL - 5
SP - 1203
EP - 1212
JO - Advanced healthcare materials
JF - Advanced healthcare materials
SN - 2192-2640
IS - 10
ER -