A bioactive metabolite of benzo[a]pyrene, benzo[a]pyrene-7,8- dione, selectively alters microsomal Ca2+ transport and ryanodine receptor function

Isaac N Pessah, Chris Beltzner, Scott W. Burchiel, Gopishetty Sridhar, Trevor Penning, Wei Feng

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Polycyclic aromatic hydrocarbons are environmental pollutants known to be carcinogenic and immunotoxic. In intact cell assays, benzo[a]pyrene (B[a]P) disrupts Ca2+ homeostasis in both immune and nonimmune cells, but the molecular mechanism is undefined. In this study, B[a]P and five metabolites are examined for their ability to alter Ca2+ transport across microsomal membranes. Using a well-defined model system, junctional SR vesicles from skeletal muscle, we show that a single o-quinone metabolite of B[a]P, B[a]P-7,8-dione, can account for altered Ca2+ transport across microsomal membranes. B[a]P-7,8-dione induces net Ca2+ release from actively loaded vesicles in a dose-, time-, and Ca2+-dependent manner. In the presence of 5 μM extravesicular Ca2+, B[a]P-7,8-dione exhibited threshold and EC50 values of 0.4 and 2 μM, respectively, and a maximal release rate of 2 μmol of Ca2+ min-1 mg-1. The mechanism by which B[a]P-7,8-dione enhanced Ca2+ efflux was further investigated by measuring macroscopic fluxes and single RyR1 channels reconstituted in bilayer lipid membranes and direct measurements of SERCA catalytic activity. B[a]P-7,8-dione (≤ 20 μM) had no measurable effect on initial rates of Ca2+ accumulation in the presence of ruthenium red to block ryanodine receptor (RyR1), nor did it alter Ca2+-dependent (thapsigarginsensitive) ATPase activity. B[a]P-7,8-dione selectively altered the function of RyR1 in a time-dependent diphasic manner, first activating then inhibiting channel activity. Considering that RyR1 and its two alternate isoforms are broadly expressed in mammalian cells and their important role in Ca2+-signaling, the present results reveal a mechanism by which metabolic bioactivation of B[a]P may mediate RyR dysfunction of pathophysiological significance.

Original languageEnglish (US)
Pages (from-to)506-513
Number of pages8
JournalMolecular Pharmacology
Volume59
Issue number3
StatePublished - 2001

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Ryanodine Receptor Calcium Release Channel
Benzo(a)pyrene
Ruthenium Red
Environmental Pollutants
Membranes
Polycyclic Aromatic Hydrocarbons
Lipid Bilayers
Membrane Lipids
Adenosine Triphosphatases
benzo(a)pyrene-7,8-dione
Protein Isoforms
Skeletal Muscle
Homeostasis

ASJC Scopus subject areas

  • Pharmacology

Cite this

A bioactive metabolite of benzo[a]pyrene, benzo[a]pyrene-7,8- dione, selectively alters microsomal Ca2+ transport and ryanodine receptor function. / Pessah, Isaac N; Beltzner, Chris; Burchiel, Scott W.; Sridhar, Gopishetty; Penning, Trevor; Feng, Wei.

In: Molecular Pharmacology, Vol. 59, No. 3, 2001, p. 506-513.

Research output: Contribution to journalArticle

Pessah, Isaac N ; Beltzner, Chris ; Burchiel, Scott W. ; Sridhar, Gopishetty ; Penning, Trevor ; Feng, Wei. / A bioactive metabolite of benzo[a]pyrene, benzo[a]pyrene-7,8- dione, selectively alters microsomal Ca2+ transport and ryanodine receptor function. In: Molecular Pharmacology. 2001 ; Vol. 59, No. 3. pp. 506-513.
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abstract = "Polycyclic aromatic hydrocarbons are environmental pollutants known to be carcinogenic and immunotoxic. In intact cell assays, benzo[a]pyrene (B[a]P) disrupts Ca2+ homeostasis in both immune and nonimmune cells, but the molecular mechanism is undefined. In this study, B[a]P and five metabolites are examined for their ability to alter Ca2+ transport across microsomal membranes. Using a well-defined model system, junctional SR vesicles from skeletal muscle, we show that a single o-quinone metabolite of B[a]P, B[a]P-7,8-dione, can account for altered Ca2+ transport across microsomal membranes. B[a]P-7,8-dione induces net Ca2+ release from actively loaded vesicles in a dose-, time-, and Ca2+-dependent manner. In the presence of 5 μM extravesicular Ca2+, B[a]P-7,8-dione exhibited threshold and EC50 values of 0.4 and 2 μM, respectively, and a maximal release rate of 2 μmol of Ca2+ min-1 mg-1. The mechanism by which B[a]P-7,8-dione enhanced Ca2+ efflux was further investigated by measuring macroscopic fluxes and single RyR1 channels reconstituted in bilayer lipid membranes and direct measurements of SERCA catalytic activity. B[a]P-7,8-dione (≤ 20 μM) had no measurable effect on initial rates of Ca2+ accumulation in the presence of ruthenium red to block ryanodine receptor (RyR1), nor did it alter Ca2+-dependent (thapsigarginsensitive) ATPase activity. B[a]P-7,8-dione selectively altered the function of RyR1 in a time-dependent diphasic manner, first activating then inhibiting channel activity. Considering that RyR1 and its two alternate isoforms are broadly expressed in mammalian cells and their important role in Ca2+-signaling, the present results reveal a mechanism by which metabolic bioactivation of B[a]P may mediate RyR dysfunction of pathophysiological significance.",
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T1 - A bioactive metabolite of benzo[a]pyrene, benzo[a]pyrene-7,8- dione, selectively alters microsomal Ca2+ transport and ryanodine receptor function

AU - Pessah, Isaac N

AU - Beltzner, Chris

AU - Burchiel, Scott W.

AU - Sridhar, Gopishetty

AU - Penning, Trevor

AU - Feng, Wei

PY - 2001

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AB - Polycyclic aromatic hydrocarbons are environmental pollutants known to be carcinogenic and immunotoxic. In intact cell assays, benzo[a]pyrene (B[a]P) disrupts Ca2+ homeostasis in both immune and nonimmune cells, but the molecular mechanism is undefined. In this study, B[a]P and five metabolites are examined for their ability to alter Ca2+ transport across microsomal membranes. Using a well-defined model system, junctional SR vesicles from skeletal muscle, we show that a single o-quinone metabolite of B[a]P, B[a]P-7,8-dione, can account for altered Ca2+ transport across microsomal membranes. B[a]P-7,8-dione induces net Ca2+ release from actively loaded vesicles in a dose-, time-, and Ca2+-dependent manner. In the presence of 5 μM extravesicular Ca2+, B[a]P-7,8-dione exhibited threshold and EC50 values of 0.4 and 2 μM, respectively, and a maximal release rate of 2 μmol of Ca2+ min-1 mg-1. The mechanism by which B[a]P-7,8-dione enhanced Ca2+ efflux was further investigated by measuring macroscopic fluxes and single RyR1 channels reconstituted in bilayer lipid membranes and direct measurements of SERCA catalytic activity. B[a]P-7,8-dione (≤ 20 μM) had no measurable effect on initial rates of Ca2+ accumulation in the presence of ruthenium red to block ryanodine receptor (RyR1), nor did it alter Ca2+-dependent (thapsigarginsensitive) ATPase activity. B[a]P-7,8-dione selectively altered the function of RyR1 in a time-dependent diphasic manner, first activating then inhibiting channel activity. Considering that RyR1 and its two alternate isoforms are broadly expressed in mammalian cells and their important role in Ca2+-signaling, the present results reveal a mechanism by which metabolic bioactivation of B[a]P may mediate RyR dysfunction of pathophysiological significance.

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