A 4,103 marker integrated physical and comparative map of the horse genome

T. Raudsepp, A. Gustafson-Seabury, K. Durkin, M. L. Wagner, G. Goh, C. M. Seabury, C. Brinkmeyer-Langford, E. J. Lee, R. Agarwala, E. Stallknecht-Rice, A. A. Schäffer, L. C. Skow, T. Tozaki, H. Yasue, Cecilia Penedo, Leslie A Lyons, K. A. Khazanehdari, M. M. Binns, J. N. MacLeod, O. DistlG. Guérin, T. Leeb, J. R. Mickelson, B. P. Chowdhary

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

A comprehensive second-generation whole genome radiation hybrid (RH II), cytogenetic and comparative map of the horse genome (2n = 64) has been developed using the 5000rad horse × hamster radiation hybrid panel and fluorescence in situ hybridization (FISH). The map contains 4,103 markers (3,816 RH; 1,144 FISH) assigned to all 31 pairs of autosomes and the X chromosome. The RH maps of individual chromosomes are anchored and oriented using 857 cytogenetic markers. The overall resolution of the map is one marker per 775 kilobase pairs (kb), which represents a more than five-fold improvement over the first-generation map. The RH II incorporates 920 markers shared jointly with the two recently reported meiotic maps. Consequently the two maps were aligned with the RH II maps of individual autosomes and the X chromosome. Additionally, a comparative map of the horse genome was generated by connecting 1,904 loci on the horse map with genome sequences available for eight diverse vertebrates to highlight regions of evolutionarily conserved syntenies, linkages, and chromosomal breakpoints. The integrated map thus obtained presents the most comprehensive information on the physical and comparative organization of the equine genome and will assist future assemblies of whole genome BAC fingerprint maps and the genome sequence. It will also serve as a tool to identify genes governing health, disease and performance traits in horses and assist us in understanding the evolution of the equine genome in relation to other species.

Original languageEnglish (US)
Pages (from-to)28-36
Number of pages9
JournalCytogenetic and Genome Research
Volume122
Issue number1
DOIs
StatePublished - Oct 2008

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Horses
Genome
Radiation Hybrid Mapping
X Chromosome
Fluorescence In Situ Hybridization
Cytogenetics
Dermatoglyphics
Cricetinae
Vertebrates
Chromosomes
Organizations
Health
Genes

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Genetics(clinical)

Cite this

Raudsepp, T., Gustafson-Seabury, A., Durkin, K., Wagner, M. L., Goh, G., Seabury, C. M., ... Chowdhary, B. P. (2008). A 4,103 marker integrated physical and comparative map of the horse genome. Cytogenetic and Genome Research, 122(1), 28-36. https://doi.org/10.1159/000151313

A 4,103 marker integrated physical and comparative map of the horse genome. / Raudsepp, T.; Gustafson-Seabury, A.; Durkin, K.; Wagner, M. L.; Goh, G.; Seabury, C. M.; Brinkmeyer-Langford, C.; Lee, E. J.; Agarwala, R.; Stallknecht-Rice, E.; Schäffer, A. A.; Skow, L. C.; Tozaki, T.; Yasue, H.; Penedo, Cecilia; Lyons, Leslie A; Khazanehdari, K. A.; Binns, M. M.; MacLeod, J. N.; Distl, O.; Guérin, G.; Leeb, T.; Mickelson, J. R.; Chowdhary, B. P.

In: Cytogenetic and Genome Research, Vol. 122, No. 1, 10.2008, p. 28-36.

Research output: Contribution to journalArticle

Raudsepp, T, Gustafson-Seabury, A, Durkin, K, Wagner, ML, Goh, G, Seabury, CM, Brinkmeyer-Langford, C, Lee, EJ, Agarwala, R, Stallknecht-Rice, E, Schäffer, AA, Skow, LC, Tozaki, T, Yasue, H, Penedo, C, Lyons, LA, Khazanehdari, KA, Binns, MM, MacLeod, JN, Distl, O, Guérin, G, Leeb, T, Mickelson, JR & Chowdhary, BP 2008, 'A 4,103 marker integrated physical and comparative map of the horse genome', Cytogenetic and Genome Research, vol. 122, no. 1, pp. 28-36. https://doi.org/10.1159/000151313
Raudsepp T, Gustafson-Seabury A, Durkin K, Wagner ML, Goh G, Seabury CM et al. A 4,103 marker integrated physical and comparative map of the horse genome. Cytogenetic and Genome Research. 2008 Oct;122(1):28-36. https://doi.org/10.1159/000151313
Raudsepp, T. ; Gustafson-Seabury, A. ; Durkin, K. ; Wagner, M. L. ; Goh, G. ; Seabury, C. M. ; Brinkmeyer-Langford, C. ; Lee, E. J. ; Agarwala, R. ; Stallknecht-Rice, E. ; Schäffer, A. A. ; Skow, L. C. ; Tozaki, T. ; Yasue, H. ; Penedo, Cecilia ; Lyons, Leslie A ; Khazanehdari, K. A. ; Binns, M. M. ; MacLeod, J. N. ; Distl, O. ; Guérin, G. ; Leeb, T. ; Mickelson, J. R. ; Chowdhary, B. P. / A 4,103 marker integrated physical and comparative map of the horse genome. In: Cytogenetic and Genome Research. 2008 ; Vol. 122, No. 1. pp. 28-36.
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