A 1.5-megabase physical map encompassing the multiple endocrine neoplasia type-1 (MEN1) locus on chromosome 11q13

Thomas F. Wood, Eri S. Srivatsan, Rita Chakrabarti, Gary C. Ma, Naikang Kuan, Ghassan J. Samara, Michael J. Higgins, Thomas B. Shows, Carey L. Johnson, Yu-Jui Yvonne Wan, Edward P. Passaro, Mark P. Sawicki

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Linkage analysis and loss of heterozygosity studies have shown that the gene responsible for the multiple endocrine neoplasia type-1 (MEN1) syndrome localizes to a small interval between D11S427 and D11S460 on chromosome 11q13. As an initial step to clone this tumor suppressor gene, our group is the first to map the MEN1 region physically using yeast artificial chromosome, bacterial artificial chromosome (BAC), and cosmid contigs. The 1.5-Mb high-resolution, contiguous map extends from PYGM to 300 kb telomeric of D11S460. Of this, the 1.2-Mb interval between PYGM and D11S460 is isolated in cosmids and BACs and will be useful for the development of genomic sequences and transcription maps of this important region. Nine new sequence-tagged sites (STS) are also characterized from this region. The physical map and the STSs will be valuable tools for the cloning of the MEN1 gene.

Original languageEnglish (US)
Pages (from-to)166-173
Number of pages8
JournalGenomics
Volume38
Issue number2
DOIs
StatePublished - Dec 1 1996

ASJC Scopus subject areas

  • Genetics

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    Wood, T. F., Srivatsan, E. S., Chakrabarti, R., Ma, G. C., Kuan, N., Samara, G. J., Higgins, M. J., Shows, T. B., Johnson, C. L., Wan, Y-J. Y., Passaro, E. P., & Sawicki, M. P. (1996). A 1.5-megabase physical map encompassing the multiple endocrine neoplasia type-1 (MEN1) locus on chromosome 11q13. Genomics, 38(2), 166-173. https://doi.org/10.1006/geno.1996.0612