A 1-month ketogenic diet increased mitochondrial mass in red gastrocnemius muscle, but not in the brain or liver of middle-aged mice

Zeyu Zhou, Jocelyn Vidales, José A. González-Reyes, Bradley Shibata, Keith Baar, Jennifer M. Rutkowsky, Jon J Ramsey

Research output: Contribution to journalArticlepeer-review

Abstract

Alterations in markers of mitochondrial content with ketogenic diets (KD) have been reported in tissues of rodents, but morphological quantification of mitochondrial mass using trans-mission electron microscopy (TEM), the gold standard for mitochondrial quantification, is needed to further validate these findings and look at specific regions of interest within a tissue. In this study, red gastrocnemius muscle, the prefrontal cortex, the hippocampus, and the liver left lobe were used to investigate the impact of a 1-month KD on mitochondrial content in healthy middle-aged mice. The results showed that in red gastrocnemius muscle, the fractional area of both subsarcolemmal (SSM) and intermyofibrillar (IMM) mitochondria was increased, and this was driven by an increase in the number of mitochondria. Mitochondrial fractional area or number was not altered in the liver, prefrontal cortex, or hippocampus following 1 month of a KD. These results demonstrate tissue-specific changes in mitochondrial mass with a short-term KD and highlight the need to study different muscle groups or tissue regions with TEM to thoroughly determine the effects of a KD on mitochon-drial mass.

Original languageEnglish (US)
Article number2533
JournalNutrients
Volume13
Issue number8
DOIs
StatePublished - Aug 2021

Keywords

  • Brain
  • Ketogenic diet
  • Liver
  • Mitochondria
  • Skeletal muscle

ASJC Scopus subject areas

  • Food Science
  • Nutrition and Dietetics

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