A γ-aminobutyric acid transporter driven by a proton pump is present in synaptic-like microvesicles of pancreatic β cells

Annette Thomas-Reetz, Johannes W Hell, Matthew J. During, Christiane Walch-Solimena, Reinhard Jahn, Pietro De Camilli

Research output: Contribution to journalArticle

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Abstract

A variety of peptide-secreting endocrine cells contain a population of recycling microvesicles that share several major membrane polypeptides with neuronal synaptic vesicles (SVs). The function of these synaptic-like microvesicles (SLMVs) remains to be elucidated. It was previously suggested that SLMVs of pancreatic aβ cells may store and secrete γ-aminobutyric acid (GABA). GABA, the major nonpeptide inhibitory neurotransmitter of the central nervous system, is stored in and secreted from SVs. GABA uptake into SVs is mediated by a transporter that is driven by a vacuolar proton ATPase. GABA is also present at high concentration in the endocrine pancreas where it is selectively localized in insulin-secreting β cells, the core cells of pancreatic islets. GABA is not present in peripheral islet cells (mantle cells), represented primarily by glucagon-secreting α cells. In this study, an immunoisolation procedure was used to purify SLMVs from cell lines derived from mouse β cells and α cells. SLMVs obtained from the β-cell line, but not those obtained from the α-cell line, displayed a GABA-transport activity dependent upon a proton electrochemical gradient generated by a vacuolar proton ATPase. These data support the hypotheses that (i) SLMVs have a secretory function similar to that of SVs and (ii) β-cell SLMVs are involved in the secretion of GABA, which in turn may have a paracrine function on mantle cells of the islet.

Original languageEnglish (US)
Pages (from-to)5317-5321
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number11
StatePublished - Jun 1 1993
Externally publishedYes

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Aminobutyrates
Proton Pumps
gamma-Aminobutyric Acid
Synaptic Vesicles
Islets of Langerhans
Vacuolar Proton-Translocating ATPases
Protons
Cell Line
Glucagon-Secreting Cells
Peptides
Endocrine Cells
Insulin-Secreting Cells
Recycling
Neurotransmitter Agents
Central Nervous System
Membranes

ASJC Scopus subject areas

  • General
  • Genetics

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A γ-aminobutyric acid transporter driven by a proton pump is present in synaptic-like microvesicles of pancreatic β cells. / Thomas-Reetz, Annette; Hell, Johannes W; During, Matthew J.; Walch-Solimena, Christiane; Jahn, Reinhard; De Camilli, Pietro.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, No. 11, 01.06.1993, p. 5317-5321.

Research output: Contribution to journalArticle

Thomas-Reetz, Annette ; Hell, Johannes W ; During, Matthew J. ; Walch-Solimena, Christiane ; Jahn, Reinhard ; De Camilli, Pietro. / A γ-aminobutyric acid transporter driven by a proton pump is present in synaptic-like microvesicles of pancreatic β cells. In: Proceedings of the National Academy of Sciences of the United States of America. 1993 ; Vol. 90, No. 11. pp. 5317-5321.
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