6-Aminonicotinamide-resistant mutants of Salmonella typhimurium

K. T. Hughes, B. T. Cookson, D. Ladika, B. M. Olivera, J. R. Roth

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Resistance to the nicotinamide analog 6-aminonicotinamide has been used to identify the following three new classes of mutants in pyridine nucleotide metabolism. pncX mutants have Tn10 insertion mutations near the pncA locus which reduce but do not eliminate the pncA product, nicotinamide deamidase. nadB (6-aminonicotinamide-resistant) mutants have dominant alleles of the nadB gene, which we propose are altered in feedback inhibition of the nadB enzyme, L-aspartate oxidase. Many of these mutants also exhibit a temperature-sensitive nicotinamide requirement phenotype. nadD mutants have mutations that affect a new gene involved in pyridine nucleotide metabolism. Since a high proportion of nadD mutations are temperature-sensitive lethal mutations, this appears to be an essential gene for NAD and NADP biosynthesis. In vivo labeling experiments indicate that in all the above cases, resistance is gained by increasing the ratio of NAD to 6-aminonicotinamide adenine dinucleotide. 6-Aminonicotinamide adenine dinucleotide turns over significantly more slowly in vivo than does normal NAD.

Original languageEnglish (US)
Pages (from-to)1126-1136
Number of pages11
JournalJournal of Bacteriology
Volume154
Issue number3
StatePublished - 1983
Externally publishedYes

Fingerprint

6-Aminonicotinamide
Salmonella typhimurium
NAD
L-aspartate oxidase
Niacinamide
Adenine
Mutation
Nicotinamidase
Nucleotides
Temperature
Insertional Mutagenesis
Essential Genes
NADP
Genes
Alleles
Phenotype
Enzymes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Hughes, K. T., Cookson, B. T., Ladika, D., Olivera, B. M., & Roth, J. R. (1983). 6-Aminonicotinamide-resistant mutants of Salmonella typhimurium. Journal of Bacteriology, 154(3), 1126-1136.

6-Aminonicotinamide-resistant mutants of Salmonella typhimurium. / Hughes, K. T.; Cookson, B. T.; Ladika, D.; Olivera, B. M.; Roth, J. R.

In: Journal of Bacteriology, Vol. 154, No. 3, 1983, p. 1126-1136.

Research output: Contribution to journalArticle

Hughes, KT, Cookson, BT, Ladika, D, Olivera, BM & Roth, JR 1983, '6-Aminonicotinamide-resistant mutants of Salmonella typhimurium', Journal of Bacteriology, vol. 154, no. 3, pp. 1126-1136.
Hughes KT, Cookson BT, Ladika D, Olivera BM, Roth JR. 6-Aminonicotinamide-resistant mutants of Salmonella typhimurium. Journal of Bacteriology. 1983;154(3):1126-1136.
Hughes, K. T. ; Cookson, B. T. ; Ladika, D. ; Olivera, B. M. ; Roth, J. R. / 6-Aminonicotinamide-resistant mutants of Salmonella typhimurium. In: Journal of Bacteriology. 1983 ; Vol. 154, No. 3. pp. 1126-1136.
@article{b59f0ffd96d84f0283221cfe0a3ba6b5,
title = "6-Aminonicotinamide-resistant mutants of Salmonella typhimurium",
abstract = "Resistance to the nicotinamide analog 6-aminonicotinamide has been used to identify the following three new classes of mutants in pyridine nucleotide metabolism. pncX mutants have Tn10 insertion mutations near the pncA locus which reduce but do not eliminate the pncA product, nicotinamide deamidase. nadB (6-aminonicotinamide-resistant) mutants have dominant alleles of the nadB gene, which we propose are altered in feedback inhibition of the nadB enzyme, L-aspartate oxidase. Many of these mutants also exhibit a temperature-sensitive nicotinamide requirement phenotype. nadD mutants have mutations that affect a new gene involved in pyridine nucleotide metabolism. Since a high proportion of nadD mutations are temperature-sensitive lethal mutations, this appears to be an essential gene for NAD and NADP biosynthesis. In vivo labeling experiments indicate that in all the above cases, resistance is gained by increasing the ratio of NAD to 6-aminonicotinamide adenine dinucleotide. 6-Aminonicotinamide adenine dinucleotide turns over significantly more slowly in vivo than does normal NAD.",
author = "Hughes, {K. T.} and Cookson, {B. T.} and D. Ladika and Olivera, {B. M.} and Roth, {J. R.}",
year = "1983",
language = "English (US)",
volume = "154",
pages = "1126--1136",
journal = "Journal of Bacteriology",
issn = "0021-9193",
publisher = "American Society for Microbiology",
number = "3",

}

TY - JOUR

T1 - 6-Aminonicotinamide-resistant mutants of Salmonella typhimurium

AU - Hughes, K. T.

AU - Cookson, B. T.

AU - Ladika, D.

AU - Olivera, B. M.

AU - Roth, J. R.

PY - 1983

Y1 - 1983

N2 - Resistance to the nicotinamide analog 6-aminonicotinamide has been used to identify the following three new classes of mutants in pyridine nucleotide metabolism. pncX mutants have Tn10 insertion mutations near the pncA locus which reduce but do not eliminate the pncA product, nicotinamide deamidase. nadB (6-aminonicotinamide-resistant) mutants have dominant alleles of the nadB gene, which we propose are altered in feedback inhibition of the nadB enzyme, L-aspartate oxidase. Many of these mutants also exhibit a temperature-sensitive nicotinamide requirement phenotype. nadD mutants have mutations that affect a new gene involved in pyridine nucleotide metabolism. Since a high proportion of nadD mutations are temperature-sensitive lethal mutations, this appears to be an essential gene for NAD and NADP biosynthesis. In vivo labeling experiments indicate that in all the above cases, resistance is gained by increasing the ratio of NAD to 6-aminonicotinamide adenine dinucleotide. 6-Aminonicotinamide adenine dinucleotide turns over significantly more slowly in vivo than does normal NAD.

AB - Resistance to the nicotinamide analog 6-aminonicotinamide has been used to identify the following three new classes of mutants in pyridine nucleotide metabolism. pncX mutants have Tn10 insertion mutations near the pncA locus which reduce but do not eliminate the pncA product, nicotinamide deamidase. nadB (6-aminonicotinamide-resistant) mutants have dominant alleles of the nadB gene, which we propose are altered in feedback inhibition of the nadB enzyme, L-aspartate oxidase. Many of these mutants also exhibit a temperature-sensitive nicotinamide requirement phenotype. nadD mutants have mutations that affect a new gene involved in pyridine nucleotide metabolism. Since a high proportion of nadD mutations are temperature-sensitive lethal mutations, this appears to be an essential gene for NAD and NADP biosynthesis. In vivo labeling experiments indicate that in all the above cases, resistance is gained by increasing the ratio of NAD to 6-aminonicotinamide adenine dinucleotide. 6-Aminonicotinamide adenine dinucleotide turns over significantly more slowly in vivo than does normal NAD.

UR - http://www.scopus.com/inward/record.url?scp=0020611251&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020611251&partnerID=8YFLogxK

M3 - Article

VL - 154

SP - 1126

EP - 1136

JO - Journal of Bacteriology

JF - Journal of Bacteriology

SN - 0021-9193

IS - 3

ER -