6-Aminonicotinamide-resistant mutants of Salmonella typhimurium

K. T. Hughes, B. T. Cookson, D. Ladika, B. M. Olivera, J. R. Roth

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Resistance to the nicotinamide analog 6-aminonicotinamide has been used to identify the following three new classes of mutants in pyridine nucleotide metabolism. pncX mutants have Tn10 insertion mutations near the pncA locus which reduce but do not eliminate the pncA product, nicotinamide deamidase. nadB (6-aminonicotinamide-resistant) mutants have dominant alleles of the nadB gene, which we propose are altered in feedback inhibition of the nadB enzyme, L-aspartate oxidase. Many of these mutants also exhibit a temperature-sensitive nicotinamide requirement phenotype. nadD mutants have mutations that affect a new gene involved in pyridine nucleotide metabolism. Since a high proportion of nadD mutations are temperature-sensitive lethal mutations, this appears to be an essential gene for NAD and NADP biosynthesis. In vivo labeling experiments indicate that in all the above cases, resistance is gained by increasing the ratio of NAD to 6-aminonicotinamide adenine dinucleotide. 6-Aminonicotinamide adenine dinucleotide turns over significantly more slowly in vivo than does normal NAD.

Original languageEnglish (US)
Pages (from-to)1126-1136
Number of pages11
JournalJournal of Bacteriology
Volume154
Issue number3
StatePublished - 1983
Externally publishedYes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

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    Hughes, K. T., Cookson, B. T., Ladika, D., Olivera, B. M., & Roth, J. R. (1983). 6-Aminonicotinamide-resistant mutants of Salmonella typhimurium. Journal of Bacteriology, 154(3), 1126-1136.