5-Series peptido-leukotriene synthesis in mouse peritoneal macrophages: Modulation by dietary n-3 fatty acids

Robert S. Chapkin, Neil Hubbard, Kent L Erickson

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

This study was undertaken to elucidate the metabolic fate of dietary eicosapentaenoic acid (20:5n-3), a major n-3 polyunsaturated fatty acid constituent of fish oil, in the mouse peritoneal macrophage. Mice were fed diets containing menhaden fish oil (FO) or safflower oil (SO). After 3 weeks, resident or responsive peritoneal macrophages were isolated and stimulated with zymosan and A23187, respectively. We demonstrate the novel synthesis of leukotriene C5 (LTC5), derived from 20:5n-3, in addition to LTC4 in both resident and responsive macrophages from FO fed mice. The structures of these peptido-leukotrienes were characterized by their retention times on reverse phase high pressure liquid chromatography, by their u.v. absorbance spectra, and by cross-reactivity with peptido-leukotriene antibody. These results demonstrate for the first time that macrophages are capable of metabolizing dietary 20:5n-3 to LTC5, a pentaene peptido-leukotriene.

Original languageEnglish (US)
Pages (from-to)764-769
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume171
Issue number2
DOIs
StatePublished - Sep 14 1990

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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