5-Aminolevulinic Acid Induces Lipid Peroxidation in Cardiolipin-Rich Liposomes

P. I. Oteiza, E. J H Bechara

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

5-Aminolevulinic acid (ALA), a heme precursor accumulated in lead poisoning and acute intermittent porphyria, is known to undergo metal-catalyzed aerobic oxidation to yield reactive oxygen species. In phosphatidylcholine:cardiolipin (80:20) liposomes ALA (0.1-3.0 mM) promoted lipid peroxidation as evaluated by the formation of conjugated dienes and 2-thiobarbituric-reactive substances (TBARS). TBARS formation was dependent on ALA concentration and incubation time. ALA-induced lipid peroxidation was associated with an increase in liposome permeability as measured by the release of encapsulated carboxyfluorescein. α-Tocopherol (0.1-0.5 mol %), an efficient oxyradical scavenger, inhibits lipid peroxidation and prevents carboxyfluorescein rejease, suggesting that the permeabilization of liposomes is mainly due to lipid peroxidation. Cardiolipin, a major component of mitochondrial inner membrane, was particularly susceptible to ALA-induced lipid peroxidation. These results may be relevant to the previously observed Ca2+-dependent permeabilization of the inner membrane of rat liver mitochondria promoted by external 0.1-1.0mM ALA; this mechanism has been implicated in the pathophysiology of acute intermittent porphyria and lead poisoning.

Original languageEnglish (US)
Pages (from-to)282-287
Number of pages6
JournalArchives of Biochemistry and Biophysics
Volume305
Issue number2
DOIs
StatePublished - Sep 1993
Externally publishedYes

Fingerprint

Aminolevulinic Acid
Cardiolipins
Liposomes
Lipid Peroxidation
Lipids
Industrial poisons
Acute Intermittent Porphyria
Lead Poisoning
Membranes
Mitochondria
Tocopherols
Liver Mitochondrion
Mitochondrial Membranes
Phosphatidylcholines
Heme
Liver
Rats
Permeability
Reactive Oxygen Species
Metals

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

5-Aminolevulinic Acid Induces Lipid Peroxidation in Cardiolipin-Rich Liposomes. / Oteiza, P. I.; Bechara, E. J H.

In: Archives of Biochemistry and Biophysics, Vol. 305, No. 2, 09.1993, p. 282-287.

Research output: Contribution to journalArticle

Oteiza, P. I. ; Bechara, E. J H. / 5-Aminolevulinic Acid Induces Lipid Peroxidation in Cardiolipin-Rich Liposomes. In: Archives of Biochemistry and Biophysics. 1993 ; Vol. 305, No. 2. pp. 282-287.
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