5-Aminolevulinic Acid Induces Iron Release from Ferritin

P. I. Oteiza, C. G. Kleinman, M. Demasi, E. J H Bechara

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

5-Aminolevulinic acid (ALA), a heme precursor accumulated in acute intermittent porphyria (AIP) and lead poisoning, undergoes metal-catalyzed oxidation in air-equilibrated solutions buffered at neutral pH, yielding free radicals (O2-, HO. and ALA.). The capacity of ALA to release iron from horse spleen and rat liver ferritin in vitro and to concomitantly initiate liposome lipid peroxidation was characterized. ALA induced iron release from ferritin in normally aerated solutions, in a dose (0.05-1 mM)- and time (0-120 min)-dependent manner; no reaction occurs under nitrogen. Superoxide dismutase partially inhibited (50% at 100 U/ml) iron release by 0.5 mM ALA, whereas the addition of catalase (50 U/ml) had no effect under these conditions. In phosphatidylcholine: cardiolipin (80:20) liposomes, and in the presence of 2 μmM EDTA, ALA (0.025-1 mM) per se had a subtle effect on lipid peroxidation, while after addition of ferritin (0.25 mg/ml) there was a significant increase in lipid peroxidation as evaluated by dose-dependent formation of 2-thiobarbituric-reactive substances and diene conjugation. In vivo, iron accumulation in the liver of ALA-treated rats was observed. Altogether, these data demonstrate the ability of ALA-generated free radicals to release iron from ferritin and to affect iron metabolism in vivo. ALA-mediated iron release from ferritin, therefore, may aggravate oxidative damage to cell components and contribute to the pathology observed in AIP (eg., primary liver cancer) and lead poisoning.

Original languageEnglish (US)
Pages (from-to)607-611
Number of pages5
JournalArchives of Biochemistry and Biophysics
Volume316
Issue number1
DOIs
StatePublished - Jan 1995
Externally publishedYes

Fingerprint

Aminolevulinic Acid
Ferritins
Iron
Industrial poisons
Liver
Lipid Peroxidation
Acute Intermittent Porphyria
Lead Poisoning
Lipids
Liposomes
Free Radicals
Rats
Cardiolipins
Pathology
Cellular Structures
Liver Neoplasms
Phosphatidylcholines
Heme
Metabolism
Edetic Acid

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

Oteiza, P. I., Kleinman, C. G., Demasi, M., & Bechara, E. J. H. (1995). 5-Aminolevulinic Acid Induces Iron Release from Ferritin. Archives of Biochemistry and Biophysics, 316(1), 607-611. https://doi.org/10.1006/abbi.1995.1080

5-Aminolevulinic Acid Induces Iron Release from Ferritin. / Oteiza, P. I.; Kleinman, C. G.; Demasi, M.; Bechara, E. J H.

In: Archives of Biochemistry and Biophysics, Vol. 316, No. 1, 01.1995, p. 607-611.

Research output: Contribution to journalArticle

Oteiza, PI, Kleinman, CG, Demasi, M & Bechara, EJH 1995, '5-Aminolevulinic Acid Induces Iron Release from Ferritin', Archives of Biochemistry and Biophysics, vol. 316, no. 1, pp. 607-611. https://doi.org/10.1006/abbi.1995.1080
Oteiza, P. I. ; Kleinman, C. G. ; Demasi, M. ; Bechara, E. J H. / 5-Aminolevulinic Acid Induces Iron Release from Ferritin. In: Archives of Biochemistry and Biophysics. 1995 ; Vol. 316, No. 1. pp. 607-611.
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