3D imaging of the vagus nerve fascicular anatomy with cryo-imaging and UV excitation

Chaitanya Kolluru, Ananya Subramaniam, Yehe Liu, Aniruddha Upadhye, Monty Khela, Lindsey Druschel, Farzad Fereidouni, Richard Levenson, Andrew Shoffstall, Michael Jenkins, David L. Wilson

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Vagus nerve stimulation (VNS) is a method to treat drug-resistant epilepsy and depression, but therapeutic outcomes are often not ideal. Newer electrode designs such as intra-fascicular electrodes offer potential improvements in reducing off-target effects but require a detailed understanding of the fascicular anatomy of the vagus nerve. We have adapted a section-and-image technique, cryo-imaging, with UV excitation to visualize fascicles along the length of the vagus nerve. In addition to offering optical sectioning at the surface via reduced penetration depth, UV illumination also produces sufficient contrast between fascicular structures and connective tissue. Here we demonstrate the utility of this approach in pilot experiments. We imaged fixed, cadaver vagus nerve samples, segmented fascicles, and demonstrated 3D tracking of fascicles. Such data can serve as input for computer models of vagus nerve stimulation.

Original languageEnglish (US)
Title of host publicationThree-Dimensional and Multidimensional Microscopy
Subtitle of host publicationImage Acquisition and Processing XXVIII
EditorsThomas G. Brown, Tony Wilson, Laura Waller
PublisherSPIE
ISBN (Electronic)9781510641334
DOIs
StatePublished - 2021
EventThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII 2021 - Virtual, Online, United States
Duration: Mar 6 2021Mar 11 2021

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume11649
ISSN (Print)1605-7422

Conference

ConferenceThree-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXVIII 2021
Country/TerritoryUnited States
CityVirtual, Online
Period3/6/213/11/21

Keywords

  • cryo-imaging
  • fascicles
  • nerve anatomy
  • vagus nerve

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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