3D anastomosed microvascular network model with living capillary networks and endothelial cell-lined microfluidic channels

Xiaolin Wang, Duc T.T. Phan, Steven George, Christopher C.W. Hughes, Abraham P. Lee

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Scopus citations

Abstract

This protocol describes detailed practical procedures for generating 3D intact and perfusable microvascular network that connects to microfluidic channels without appreciable leakage. This advanced 3D microvascular network model incorporates different stages of vascular development including vasculogenesis, endothelial cell (EC) lining, sprouting angiogenesis, and anastomosis in sequential order. The capillary network is first induced via vasculogenesis in a middle tissue chamber and then EC linings along the microfluidic channel on either side serve as artery and vein. The anastomosis is then induced by sprouting angiogenesis to facilitate tight interconnection between the artery/vein and the capillary network. This versatile device design and its robust construction methodology establish a physiological microcirculation transport model of interconnected perfused vessels from artery to vascularized tissue to vein.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages325-344
Number of pages20
DOIs
StatePublished - Jan 1 2017
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1612
ISSN (Print)1064-3745

Keywords

  • 3D microvascular network
  • Anastomosis
  • EC lining
  • Microfluidic chip
  • Non-physiological leakage
  • Organ-on-a-chip
  • Sprouting angiogenesis
  • Vasculogenesis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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  • Cite this

    Wang, X., Phan, D. T. T., George, S., Hughes, C. C. W., & Lee, A. P. (2017). 3D anastomosed microvascular network model with living capillary networks and endothelial cell-lined microfluidic channels. In Methods in Molecular Biology (pp. 325-344). (Methods in Molecular Biology; Vol. 1612). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-7021-6_24