2,3,7,8-Tetrachlorodibenzo-p-dioxin induces CYP1B1 expression in human luteinized granulosa cells

J. D. Vidal, C. A. VandeVoort, C. B. Marcus, N. R. Lazarewicz, Alan J Conley

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

The environmental contaminant 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is a reproductive toxicant in multiple species; however, mechanisms and direct ovarian effects are poorly understood. DNA microarrays were used to characterize gene expression profiles of human luteinized granulosa cells (HLGCs) exposed to TCDD in primary cultures. Exposure to 10 nM TCDD for 24 h induced a significant increase in CYP1B1, while few other genes responded. TaqMan PCR and Western immunoblotting demonstrated that induction was dose-dependent. Additionally, the microsomal form of catechol-O-methyltransferase (COMT) was highly expressed in HLGCs, along with only fractional amounts of the soluble form. This is the first report of CYP1B1 and COMT expression, and CYP1B1 induction, in cells from the human ovary. The role of CYP1B1 in the oxidative metabolism of estrogens and potential generation of DNA adducts in the ovary may have significant consequences for oocyte quality, corpus luteum function, and ovarian carcinogenesis.

Original languageEnglish (US)
Pages (from-to)53-60
Number of pages8
JournalArchives of Biochemistry and Biophysics
Volume439
Issue number1
DOIs
StatePublished - Jul 1 2005

Keywords

  • 2,3,7,8-Tetrachlorodibenzo-p-dioxin
  • Catechol-O-methyltransferase
  • Corpus luteum
  • CYP1B1
  • Microarrays
  • Ovary

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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