20Hepatoprotective effect of C-phycocyanin: Protection for carbon tetrachloride and R-(+)-pulegone-mediated hepatotoxicty in rats

Bhat B. Vadiraja, Nilesh W. Gaikwad, K. M. Madyastha

Research output: Contribution to journalArticle

147 Citations (Scopus)

Abstract

Effect of C-phycocyanin (from Spirulina platensis) pretreatment on carbontetrachloride and R-(+)-pulegone-induced hepatotoxicity in rats was studied. Intraperitoneal (i.p.) administration (200 mg/kg) of a single dose of phycocyanin to rats, one or three hours prior to R-(+)-pulegone (250 mg/kg) or carbontetrachloride (0.6 ml/kg) challenge, significantly reduced the hepatotoxicity caused by these chemicals. For instance, serum glutamate pyruvate transaminase (SGPT) activity was almost equal to control values. The losses of microsomal cytochrome P450, glucose-6-phosphatase and aminopyrine-N-demethylase were significantly reduced, suggesting that phycocyanin provides protection to liver enzymes. It was noticed that the level of menthofuran, the proximate toxin of R-(+)-pulegone was nearly 70% more in the urine samples collected from rats treated with R-(+)-pulegone alone than rats treated with the combination of phycocyanin and R-(+)-pulegone. The possible mechanism involved in the hepatoprotection is discussed.

Original languageEnglish (US)
Pages (from-to)428-431
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume249
Issue number2
DOIs
StatePublished - Aug 19 1998
Externally publishedYes

Fingerprint

Phycocyanin
Carbon Tetrachloride
Rats
Aminopyrine N-Demethylase
Spirulina
Glucose-6-Phosphatase
Transaminases
Pyruvic Acid
Liver
Cytochrome P-450 Enzyme System
Glutamic Acid
pulegone
Urine
Enzymes
Serum

Keywords

  • A monoterpene ketone
  • C-phycocyanin
  • Carbon tetrachloride
  • Hepatoprotective effect
  • Hepatotoxicity
  • R-(+)-pulegone
  • Spirulina platensis

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

20Hepatoprotective effect of C-phycocyanin : Protection for carbon tetrachloride and R-(+)-pulegone-mediated hepatotoxicty in rats. / Vadiraja, Bhat B.; Gaikwad, Nilesh W.; Madyastha, K. M.

In: Biochemical and Biophysical Research Communications, Vol. 249, No. 2, 19.08.1998, p. 428-431.

Research output: Contribution to journalArticle

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abstract = "Effect of C-phycocyanin (from Spirulina platensis) pretreatment on carbontetrachloride and R-(+)-pulegone-induced hepatotoxicity in rats was studied. Intraperitoneal (i.p.) administration (200 mg/kg) of a single dose of phycocyanin to rats, one or three hours prior to R-(+)-pulegone (250 mg/kg) or carbontetrachloride (0.6 ml/kg) challenge, significantly reduced the hepatotoxicity caused by these chemicals. For instance, serum glutamate pyruvate transaminase (SGPT) activity was almost equal to control values. The losses of microsomal cytochrome P450, glucose-6-phosphatase and aminopyrine-N-demethylase were significantly reduced, suggesting that phycocyanin provides protection to liver enzymes. It was noticed that the level of menthofuran, the proximate toxin of R-(+)-pulegone was nearly 70{\%} more in the urine samples collected from rats treated with R-(+)-pulegone alone than rats treated with the combination of phycocyanin and R-(+)-pulegone. The possible mechanism involved in the hepatoprotection is discussed.",
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