17-β-estradiol induces heat shock proteins in brain arteries and potentiates ischemic heat shock protein induction in glia and neurons

Aigang Lu, Rui Qiong Ran, Joseph Clark, Melinda Reilly, Alex Nee, Frank R Sharp

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Estradiol reduces brain injury from many diseases, including stroke and trauma. To investigate the molecular mechanisms of this protection, the effects of 17-β-estradiol on heat shock protein (HSP) expression were studied in normal male and female rats and in male gerbils after global ischemia. 17-β-Estradiol was given intraperitoneally (46 or 460 ng/kg, or 4.6 μg/kg) and Western blots performed for HSPs. 17-β-Estradiol increased hemeoxygenase-1, HSP25/27, and HSP70 in the brain of male and female rats. Six hours after the administration of 17-β-estradiol, hemeoxygenase-1 increased 3.9-fold (460 ng/kg) and 5.4-fold (4.6 μg/kg), HSP25/27 increased 2.1-fold (4.6 μg/kg), and Hsp70 increased 2.3-fold (460 ng/kg). Immunocytochemistry showed that hemeoxygenase-1, HSP25/27, and HSP70 induction was localized to cerebral arteries in male rats, possibly in vascular smooth muscle cells. 17-β-Estradiol was injected intraperitoneally 20 minutes before transient occlusion of both carotids in adult gerbils. Six hours after global cerebral ischemia, 17-β-estradiol (460 ng/kg) increased levels of hemeoxygenase-1 protein 2.4-fold compared with ischemia alone, and HSP25/27 levels increased 1.8-fold compared with ischemia alone. Hemeoxygenase-1 was induced in striatal oligodendrocytes and hippocampal neurons, and HSP25/27 levels increased in striatal astrocytes and hippocampal neurons. Finally, Western blot analysis confirmed that estrogen induced heat shock factor-1, providing a possible mechanism by which estrogen induces HSPs in brain and other tissues. The induction of HSPs may be an important mechanism for estrogen protection against cerebral ischemia and other types of injury.

Original languageEnglish (US)
Pages (from-to)183-195
Number of pages13
JournalJournal of Cerebral Blood Flow and Metabolism
Volume22
Issue number2
StatePublished - 2002
Externally publishedYes

Fingerprint

Heat-Shock Proteins
Neuroglia
Heme Oxygenase-1
Estradiol
Arteries
Neurons
Brain
Corpus Striatum
Estrogens
Ischemia
Gerbillinae
Brain Ischemia
Western Blotting
Cerebral Arteries
Oligodendroglia
Wounds and Injuries
Vascular Smooth Muscle
Astrocytes
Brain Injuries
Smooth Muscle Myocytes

Keywords

  • Arteries
  • Blood vessels
  • Estrogen
  • Heat shock
  • Heat shock factor
  • Heat shock proteins
  • Ischemia
  • Smooth muscle cells
  • Stroke

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)
  • Endocrinology, Diabetes and Metabolism

Cite this

17-β-estradiol induces heat shock proteins in brain arteries and potentiates ischemic heat shock protein induction in glia and neurons. / Lu, Aigang; Ran, Rui Qiong; Clark, Joseph; Reilly, Melinda; Nee, Alex; Sharp, Frank R.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 22, No. 2, 2002, p. 183-195.

Research output: Contribution to journalArticle

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T1 - 17-β-estradiol induces heat shock proteins in brain arteries and potentiates ischemic heat shock protein induction in glia and neurons

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AU - Nee, Alex

AU - Sharp, Frank R

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N2 - Estradiol reduces brain injury from many diseases, including stroke and trauma. To investigate the molecular mechanisms of this protection, the effects of 17-β-estradiol on heat shock protein (HSP) expression were studied in normal male and female rats and in male gerbils after global ischemia. 17-β-Estradiol was given intraperitoneally (46 or 460 ng/kg, or 4.6 μg/kg) and Western blots performed for HSPs. 17-β-Estradiol increased hemeoxygenase-1, HSP25/27, and HSP70 in the brain of male and female rats. Six hours after the administration of 17-β-estradiol, hemeoxygenase-1 increased 3.9-fold (460 ng/kg) and 5.4-fold (4.6 μg/kg), HSP25/27 increased 2.1-fold (4.6 μg/kg), and Hsp70 increased 2.3-fold (460 ng/kg). Immunocytochemistry showed that hemeoxygenase-1, HSP25/27, and HSP70 induction was localized to cerebral arteries in male rats, possibly in vascular smooth muscle cells. 17-β-Estradiol was injected intraperitoneally 20 minutes before transient occlusion of both carotids in adult gerbils. Six hours after global cerebral ischemia, 17-β-estradiol (460 ng/kg) increased levels of hemeoxygenase-1 protein 2.4-fold compared with ischemia alone, and HSP25/27 levels increased 1.8-fold compared with ischemia alone. Hemeoxygenase-1 was induced in striatal oligodendrocytes and hippocampal neurons, and HSP25/27 levels increased in striatal astrocytes and hippocampal neurons. Finally, Western blot analysis confirmed that estrogen induced heat shock factor-1, providing a possible mechanism by which estrogen induces HSPs in brain and other tissues. The induction of HSPs may be an important mechanism for estrogen protection against cerebral ischemia and other types of injury.

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