17β-Estradiol reduces glycoxidative damage in the artery wall

Barbara A. Walsh, Bonnie L. Busch, Adam E. Mullick, Karen M. Reiser, John C Rutledge

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Glycoxidative damage in the vasculature has been linked to atherosclerotic cardiovascular disease. Estrogens protect against the development and progression of atherosclerosis. Because estrogens are potent antioxidants that also effect glucose metabolism, part of their protection against atherosclerosis could be through attenuation of glycoxidative damage in the vascular wall. In this study, we tested the hypothesis that chronic estradiol administration is associated with decreased levels of glycoxidative damage in arterial walls. We harvested and examined iliac arteries from ovariectomized, 8-month-old rats that had been implanted for 6 months with 1 of the following subcutaneous hormone pellets: low estradiol (2.5 mg estradiol), high estradiol (25 mg estradiol), P4 (200 mg progesterone), low estradiol and P4, placebo (no hormone), or control (no implant). Using pentosidine as a biomarker of glycoxidative damage, we found that all vessels from rats receiving estradiol (low estradiol, high estradiol, and low estradiol+P4) exhibited a 50% reduction in glycoxidative damage compared with P4, placebo, and control vessels (P<0.05). Consistent with this finding, we observed that estradiol-treated rats had a 30% decrease in tissue levels of hydroperoxides, a marker of oxidative stress. Finally, estradiol- treated rats had a small, but significant, decrease in plasma glucose levels (P<0.01). In summary, we report the novel finding that chronic estrogen administration is associated with significant decreases in glycoxidative damage and oxidative stress in the arterial wall. It seems likely that these actions may constitute a mechanism by which estrogen attenuates the progression of atherosclerosis.

Original languageEnglish (US)
Pages (from-to)840-846
Number of pages7
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume19
Issue number4
StatePublished - Apr 1999

Fingerprint

Estradiol
Arteries
Estrogens
Atherosclerosis
Oxidative Stress
Placebos
Hormones
Glucose
Iliac Artery
Hydrogen Peroxide
Progesterone
Blood Vessels
Cardiovascular Diseases
Antioxidants
Biomarkers

Keywords

  • Atherosclerosis
  • Estrogen
  • Glycemic stress
  • Glycoxidative damage
  • Oxidant stress

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

17β-Estradiol reduces glycoxidative damage in the artery wall. / Walsh, Barbara A.; Busch, Bonnie L.; Mullick, Adam E.; Reiser, Karen M.; Rutledge, John C.

In: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 19, No. 4, 04.1999, p. 840-846.

Research output: Contribution to journalArticle

Walsh, BA, Busch, BL, Mullick, AE, Reiser, KM & Rutledge, JC 1999, '17β-Estradiol reduces glycoxidative damage in the artery wall', Arteriosclerosis, Thrombosis, and Vascular Biology, vol. 19, no. 4, pp. 840-846.
Walsh, Barbara A. ; Busch, Bonnie L. ; Mullick, Adam E. ; Reiser, Karen M. ; Rutledge, John C. / 17β-Estradiol reduces glycoxidative damage in the artery wall. In: Arteriosclerosis, Thrombosis, and Vascular Biology. 1999 ; Vol. 19, No. 4. pp. 840-846.
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