[13] Purification of novel kinesins from embryonic systems

David Meyer, Daniel R. Rines, Anna Kashina, Douglas G. Cole, Jonathan M. Scholey

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Several kinesin holoenzymes, including the heterotrimeric kinesin-II and bipolar KLP61F complexes described here, are being purified in our laboratory using microtubule affinity precipitation and conventional biochemical fractionation procedures.4 These protocols have been optimized by using pan- kinesin peptide antibodies and subunit-specific antibodies to monitor the enrichment of kinesin-related polypeptides in particular fractions by immunoblotting. Protein purification represent the most direct route available for determining the oligomeric state and subunit composition of a kinesin holoenzyme, for identifying tightly associated accessory subunits such as SpKAP115, and for determining the molecular architecture and functional properties of native kinesin motors.49 Protein purification methods therefore represent an important complementary approach to molecular genetic approaches that are being pursued in many other laboratories.

Original languageEnglish (US)
Pages (from-to)133-154
Number of pages22
JournalMethods in Enzymology
Volume298
DOIs
StatePublished - 1998
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry

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    Meyer, D., Rines, D. R., Kashina, A., Cole, D. G., & Scholey, J. M. (1998). [13] Purification of novel kinesins from embryonic systems. Methods in Enzymology, 298, 133-154. https://doi.org/10.1016/S0076-6879(98)98015-6