1,25-Dihydroxyvitamin D3 increases collagen production in dermal fibroblasts

John Dobak, Jacek Grzybowski, Fu-Tong Liu, Bruce Landon, Marek Dobke

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

The effect of 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) on proliferation and collagen type I and type III production in cultured human fibroblasts was examined. Previous studies have identified receptors for the vitamin in human dermal fibroblasts, and have suggested the skin to be a target tissue. While many studies examining keratinocyte modulation by 1,25-(OH)2D3 have been undertaken, very few have been performed on dermal fibroblasts. Neonatal foreskin fibroblast cultures were examined for cell number, extracellular collagen accumulation, and collagen mRNA levels, after 5 days exposure to 1,25-(OH)2D3 at a concentration of 10-7 M. The vitamin significantly suppressed (P < 0.01) the proliferation of fibroblasts cultured in the presence of serum. Day 5 cell culture supernatants showed a significant per cell increase in collagen type I (P < 0.05) and type III (P < 0.01) as measured by ELISA. Type I collagen production in exposed cells was 11.64 + 0.531 μg/106 cell vs. 9.53 + 0.500 μg/106 cells in unexposed cells. Type III collagen production was 0.601 + 0.012 μg/106 cell in exposed cells and 0.247 + 0.008 μg/106 cells in unexposed cells. mRNA levels were increased after a 4-day exposure to 10-7 M 1,25-(OH)2D3 for both type I (2.5-5-fold) and type III (5.5-7.76-fold) collagen. These results suggest a novel effect of increased collagen production by dermal fibroblasts upon exposure to 1,25-(OH)2D3 that is independent of proliferation.

Original languageEnglish (US)
Pages (from-to)18-24
Number of pages7
JournalJournal of Dermatological Science
Volume8
Issue number1
DOIs
StatePublished - 1994

Keywords

  • Collagen production
  • Dermal fibroblasts

ASJC Scopus subject areas

  • Dermatology

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