Abstract
Since interferons have been shown to affect the synthesis of matrix proteins such as collagen in several in vitro systems, the potential role of γ-interferon in inhibiting hepatic fibrosis was investigated. Hepatic cells, consisting primarily of hepatocytes, were treated with recombinant γ-interferon for 24 hr. Northern blot hybridization showed that γ-interferon treatment caused a profound decrease in pro-α2(I)collagen mRNA levels but an increase in β-actin mRNA content. The effects of γ-interferon were then studied in an in vivo model of hepatic fibrogenesis, murine schistosomiasis. Schistosoma-infected mice were treated with daily i.m. injections of γ-interferon for a 4-week period starting 4 weeks after the initial infection. γ-Interferon treatment decreased collagen deposition as determined by histologic evaluation and measurement of total liver collagen content. Northern blots showed Types I and III procollagen mRNA levels for treated, infected animals to be only 32 and 29% that of infected controls, but β-actin mRNA levels were significantly elevated. These results indicate a potential role for γ-interferon as an antifibrogenic agent in vivo.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 795-800 |
| Number of pages | 6 |
| Journal | Hepatology |
| Volume | 10 |
| Issue number | 5 |
| State | Published - 1989 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Hepatology
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γ-Interferon treatment inhibits collagen deposition in murine schistosomiasis. / Czaja, M. J.; Weiner, F. R.; Takahashi, S.; Giambrone, M. A.; Van der Meide, P. H.; Schellekens, H.; Biempica, L.; Zern, Mark A.
In: Hepatology, Vol. 10, No. 5, 1989, p. 795-800.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - γ-Interferon treatment inhibits collagen deposition in murine schistosomiasis
AU - Czaja, M. J.
AU - Weiner, F. R.
AU - Takahashi, S.
AU - Giambrone, M. A.
AU - Van der Meide, P. H.
AU - Schellekens, H.
AU - Biempica, L.
AU - Zern, Mark A
PY - 1989
Y1 - 1989
N2 - Since interferons have been shown to affect the synthesis of matrix proteins such as collagen in several in vitro systems, the potential role of γ-interferon in inhibiting hepatic fibrosis was investigated. Hepatic cells, consisting primarily of hepatocytes, were treated with recombinant γ-interferon for 24 hr. Northern blot hybridization showed that γ-interferon treatment caused a profound decrease in pro-α2(I)collagen mRNA levels but an increase in β-actin mRNA content. The effects of γ-interferon were then studied in an in vivo model of hepatic fibrogenesis, murine schistosomiasis. Schistosoma-infected mice were treated with daily i.m. injections of γ-interferon for a 4-week period starting 4 weeks after the initial infection. γ-Interferon treatment decreased collagen deposition as determined by histologic evaluation and measurement of total liver collagen content. Northern blots showed Types I and III procollagen mRNA levels for treated, infected animals to be only 32 and 29% that of infected controls, but β-actin mRNA levels were significantly elevated. These results indicate a potential role for γ-interferon as an antifibrogenic agent in vivo.
AB - Since interferons have been shown to affect the synthesis of matrix proteins such as collagen in several in vitro systems, the potential role of γ-interferon in inhibiting hepatic fibrosis was investigated. Hepatic cells, consisting primarily of hepatocytes, were treated with recombinant γ-interferon for 24 hr. Northern blot hybridization showed that γ-interferon treatment caused a profound decrease in pro-α2(I)collagen mRNA levels but an increase in β-actin mRNA content. The effects of γ-interferon were then studied in an in vivo model of hepatic fibrogenesis, murine schistosomiasis. Schistosoma-infected mice were treated with daily i.m. injections of γ-interferon for a 4-week period starting 4 weeks after the initial infection. γ-Interferon treatment decreased collagen deposition as determined by histologic evaluation and measurement of total liver collagen content. Northern blots showed Types I and III procollagen mRNA levels for treated, infected animals to be only 32 and 29% that of infected controls, but β-actin mRNA levels were significantly elevated. These results indicate a potential role for γ-interferon as an antifibrogenic agent in vivo.
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UR - http://www.scopus.com/inward/citedby.url?scp=0024416580&partnerID=8YFLogxK
M3 - Article
C2 - 2509321
AN - SCOPUS:0024416580
VL - 10
SP - 795
EP - 800
JO - Hepatology
JF - Hepatology
SN - 0270-9139
IS - 5
ER -