γ-Interferon treatment inhibits collagen deposition in murine schistosomiasis

M. J. Czaja, F. R. Weiner, S. Takahashi, M. A. Giambrone, P. H. Van der Meide, H. Schellekens, L. Biempica, Mark A Zern

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Abstract

Since interferons have been shown to affect the synthesis of matrix proteins such as collagen in several in vitro systems, the potential role of γ-interferon in inhibiting hepatic fibrosis was investigated. Hepatic cells, consisting primarily of hepatocytes, were treated with recombinant γ-interferon for 24 hr. Northern blot hybridization showed that γ-interferon treatment caused a profound decrease in pro-α2(I)collagen mRNA levels but an increase in β-actin mRNA content. The effects of γ-interferon were then studied in an in vivo model of hepatic fibrogenesis, murine schistosomiasis. Schistosoma-infected mice were treated with daily i.m. injections of γ-interferon for a 4-week period starting 4 weeks after the initial infection. γ-Interferon treatment decreased collagen deposition as determined by histologic evaluation and measurement of total liver collagen content. Northern blots showed Types I and III procollagen mRNA levels for treated, infected animals to be only 32 and 29% that of infected controls, but β-actin mRNA levels were significantly elevated. These results indicate a potential role for γ-interferon as an antifibrogenic agent in vivo.

Original languageEnglish (US)
Pages (from-to)795-800
Number of pages6
JournalHepatology
Volume10
Issue number5
StatePublished - 1989
Externally publishedYes

Fingerprint

Schistosomiasis
Interferons
Collagen
Messenger RNA
Therapeutics
Northern Blotting
Actins
Hepatocytes
Liver
Schistosoma
Collagen Type III
Fibrosis
Injections
Infection

ASJC Scopus subject areas

  • Hepatology

Cite this

Czaja, M. J., Weiner, F. R., Takahashi, S., Giambrone, M. A., Van der Meide, P. H., Schellekens, H., ... Zern, M. A. (1989). γ-Interferon treatment inhibits collagen deposition in murine schistosomiasis. Hepatology, 10(5), 795-800.

γ-Interferon treatment inhibits collagen deposition in murine schistosomiasis. / Czaja, M. J.; Weiner, F. R.; Takahashi, S.; Giambrone, M. A.; Van der Meide, P. H.; Schellekens, H.; Biempica, L.; Zern, Mark A.

In: Hepatology, Vol. 10, No. 5, 1989, p. 795-800.

Research output: Contribution to journalArticle

Czaja, MJ, Weiner, FR, Takahashi, S, Giambrone, MA, Van der Meide, PH, Schellekens, H, Biempica, L & Zern, MA 1989, 'γ-Interferon treatment inhibits collagen deposition in murine schistosomiasis', Hepatology, vol. 10, no. 5, pp. 795-800.
Czaja MJ, Weiner FR, Takahashi S, Giambrone MA, Van der Meide PH, Schellekens H et al. γ-Interferon treatment inhibits collagen deposition in murine schistosomiasis. Hepatology. 1989;10(5):795-800.
Czaja, M. J. ; Weiner, F. R. ; Takahashi, S. ; Giambrone, M. A. ; Van der Meide, P. H. ; Schellekens, H. ; Biempica, L. ; Zern, Mark A. / γ-Interferon treatment inhibits collagen deposition in murine schistosomiasis. In: Hepatology. 1989 ; Vol. 10, No. 5. pp. 795-800.
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