β2-adrenergic receptor supports prolonged theta tetanus-induced LTP

Hai Qian, Lucas Matt, Mingxu Zhang, Minh Nguyen, Tommaso Patriarchi, Olha M. Koval, Mark E. Anderson, Kaiwen He, Hey Kyoung Lee, Johannes W Hell

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

The Widespread noradrenergic innervations in the brain promotes arousal and learning by molecular mechanisms that remain largely undefined. Recent work shows that the β2-adrenergic receptor (β2AR) is linked to the AMPA-type glutamate receptor subunit GluA1 via stargazin and PSD-95 (Joiner ML, Lise MF, Yuen EY, Kam AY, Zhang M, Hall DD, Malik ZA, Qian H, Chen Y, Ulrich JD, Burette AC, Weinberg RJ, Law PY, El-Husseini A, Yan Z, Hell JW. EMBO J 29: 482-495, 2010). We now demonstrate that the β2AR plays a prominent role in long-term potentiation (LTP) induced by a train of 900 stimuli at 5 Hz (prolonged theta-tetanus-LTP, or PTT-LTP) in the hippocampal CA1 region in mice, which requires simultaneous β -adrenergic stimulation. Although PTT-LTP was impaired in hippocampal slices from β1AR and β 2AR knockout (KO) mice, only β 2AR-selective stimulation with salbutamol supported this PTT-LTP in wild-type (WT) slices, whereas β1AR-selective stimulation with dobutamine (+ prazosin) did not. Furthermore, only the β 2AR-selective antagonist ICI-118551 and not the β1AR-selective antagonist CGP-20712 inhibited PTT-LTP and phosphorylation of GluA1 on its PKA site S845 in WT slices. Our analysis of S845A knockin (KI) mice indicates that this phosphorylation is relevant for PTT-LTP. These results identify the β 2AR-S845 signaling pathway as a prominent regulator of synaptic plasticity.

Original languageEnglish (US)
Pages (from-to)2703-2712
Number of pages10
JournalJournal of Neurophysiology
Volume107
Issue number10
DOIs
StatePublished - May 15 2012

Fingerprint

Long-Term Potentiation
Tetanus
Adrenergic Receptors
Phosphorylation
Hippocampal CA1 Region
Neuronal Plasticity
Adrenergic Antagonists
Dobutamine
AMPA Receptors
Albuterol
Prazosin
Glutamate Receptors
Arousal
Knockout Mice
Adrenergic Agents
Learning
Brain

Keywords

  • α3-hy-droxy-5-methylisoxazole propionic acid receptors
  • Beta-adrenergic signaling
  • Postsynaptic signaling
  • Protein kinase A

ASJC Scopus subject areas

  • Physiology
  • Neuroscience(all)

Cite this

Qian, H., Matt, L., Zhang, M., Nguyen, M., Patriarchi, T., Koval, O. M., ... Hell, J. W. (2012). β2-adrenergic receptor supports prolonged theta tetanus-induced LTP. Journal of Neurophysiology, 107(10), 2703-2712. https://doi.org/10.1152/jn.00374.2011

β2-adrenergic receptor supports prolonged theta tetanus-induced LTP. / Qian, Hai; Matt, Lucas; Zhang, Mingxu; Nguyen, Minh; Patriarchi, Tommaso; Koval, Olha M.; Anderson, Mark E.; He, Kaiwen; Lee, Hey Kyoung; Hell, Johannes W.

In: Journal of Neurophysiology, Vol. 107, No. 10, 15.05.2012, p. 2703-2712.

Research output: Contribution to journalArticle

Qian, H, Matt, L, Zhang, M, Nguyen, M, Patriarchi, T, Koval, OM, Anderson, ME, He, K, Lee, HK & Hell, JW 2012, 'β2-adrenergic receptor supports prolonged theta tetanus-induced LTP', Journal of Neurophysiology, vol. 107, no. 10, pp. 2703-2712. https://doi.org/10.1152/jn.00374.2011
Qian H, Matt L, Zhang M, Nguyen M, Patriarchi T, Koval OM et al. β2-adrenergic receptor supports prolonged theta tetanus-induced LTP. Journal of Neurophysiology. 2012 May 15;107(10):2703-2712. https://doi.org/10.1152/jn.00374.2011
Qian, Hai ; Matt, Lucas ; Zhang, Mingxu ; Nguyen, Minh ; Patriarchi, Tommaso ; Koval, Olha M. ; Anderson, Mark E. ; He, Kaiwen ; Lee, Hey Kyoung ; Hell, Johannes W. / β2-adrenergic receptor supports prolonged theta tetanus-induced LTP. In: Journal of Neurophysiology. 2012 ; Vol. 107, No. 10. pp. 2703-2712.
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