β1 integrin expression pattern in transitional urothelium does not allow for efficient stem cell enrichment as in other epithelia

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Abstract

With a lack of distinct stem cell markers, isolation of tissue-specific stem cells for tissue engineering and gene therapy is a great challenge. Beta (β)1 integrin expression has been used as a way of enriching for putative epithelial stem cells through rapid adhesion to collagen IV or flow cytometry. This is a first report of enrichment of putative urothelial stem cells using rapid adhesion and flow cytometric methods. We assessed our success by determining the clonogenic and proliferative potential of the isolated cells. We demonstrated that enrichment based on β1 integrin expression with flow cytometry yields highly clonogenic and proliferative urothelial cells, whereas the rapid adhesion method is not as efficient, possibly because of the unique nature of urothelium, a transitional epithelium, compared to results reported in stratified and columnar epithelia.

Original languageEnglish (US)
Pages (from-to)263-270
Number of pages8
JournalTissue Engineering
Volume13
Issue number2
DOIs
StatePublished - Feb 2007

Fingerprint

Urothelium
Stem cells
Integrins
Stem Cells
Epithelium
Adhesion
Flow cytometry
Flow Cytometry
Integrin beta Chains
Cell Engineering
Gene therapy
Cell Separation
Tissue Engineering
Cell- and Tissue-Based Therapy
Tissue engineering
Collagen
Genetic Therapy
Epithelial Cells
Tissue

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology
  • Biotechnology

Cite this

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title = "β1 integrin expression pattern in transitional urothelium does not allow for efficient stem cell enrichment as in other epithelia",
abstract = "With a lack of distinct stem cell markers, isolation of tissue-specific stem cells for tissue engineering and gene therapy is a great challenge. Beta (β)1 integrin expression has been used as a way of enriching for putative epithelial stem cells through rapid adhesion to collagen IV or flow cytometry. This is a first report of enrichment of putative urothelial stem cells using rapid adhesion and flow cytometric methods. We assessed our success by determining the clonogenic and proliferative potential of the isolated cells. We demonstrated that enrichment based on β1 integrin expression with flow cytometry yields highly clonogenic and proliferative urothelial cells, whereas the rapid adhesion method is not as efficient, possibly because of the unique nature of urothelium, a transitional epithelium, compared to results reported in stratified and columnar epithelia.",
author = "Deborah Lieu and Degraffenried, {Lea A.} and Isseroff, {Roslyn Rivkah} and Kurzrock, {Eric A}",
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T1 - β1 integrin expression pattern in transitional urothelium does not allow for efficient stem cell enrichment as in other epithelia

AU - Lieu, Deborah

AU - Degraffenried, Lea A.

AU - Isseroff, Roslyn Rivkah

AU - Kurzrock, Eric A

PY - 2007/2

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AB - With a lack of distinct stem cell markers, isolation of tissue-specific stem cells for tissue engineering and gene therapy is a great challenge. Beta (β)1 integrin expression has been used as a way of enriching for putative epithelial stem cells through rapid adhesion to collagen IV or flow cytometry. This is a first report of enrichment of putative urothelial stem cells using rapid adhesion and flow cytometric methods. We assessed our success by determining the clonogenic and proliferative potential of the isolated cells. We demonstrated that enrichment based on β1 integrin expression with flow cytometry yields highly clonogenic and proliferative urothelial cells, whereas the rapid adhesion method is not as efficient, possibly because of the unique nature of urothelium, a transitional epithelium, compared to results reported in stratified and columnar epithelia.

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