Abstract
With a lack of distinct stem cell markers, isolation of tissue-specific stem cells for tissue engineering and gene therapy is a great challenge. Beta (β)1 integrin expression has been used as a way of enriching for putative epithelial stem cells through rapid adhesion to collagen IV or flow cytometry. This is a first report of enrichment of putative urothelial stem cells using rapid adhesion and flow cytometric methods. We assessed our success by determining the clonogenic and proliferative potential of the isolated cells. We demonstrated that enrichment based on β1 integrin expression with flow cytometry yields highly clonogenic and proliferative urothelial cells, whereas the rapid adhesion method is not as efficient, possibly because of the unique nature of urothelium, a transitional epithelium, compared to results reported in stratified and columnar epithelia.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 263-270 |
| Number of pages | 8 |
| Journal | Tissue Engineering |
| Volume | 13 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 2007 |
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ASJC Scopus subject areas
- Biophysics
- Cell Biology
- Biotechnology
Cite this
β1 integrin expression pattern in transitional urothelium does not allow for efficient stem cell enrichment as in other epithelia. / Lieu, Deborah; Degraffenried, Lea A.; Isseroff, Roslyn Rivkah; Kurzrock, Eric A.
In: Tissue Engineering, Vol. 13, No. 2, 02.2007, p. 263-270.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - β1 integrin expression pattern in transitional urothelium does not allow for efficient stem cell enrichment as in other epithelia
AU - Lieu, Deborah
AU - Degraffenried, Lea A.
AU - Isseroff, Roslyn Rivkah
AU - Kurzrock, Eric A
PY - 2007/2
Y1 - 2007/2
N2 - With a lack of distinct stem cell markers, isolation of tissue-specific stem cells for tissue engineering and gene therapy is a great challenge. Beta (β)1 integrin expression has been used as a way of enriching for putative epithelial stem cells through rapid adhesion to collagen IV or flow cytometry. This is a first report of enrichment of putative urothelial stem cells using rapid adhesion and flow cytometric methods. We assessed our success by determining the clonogenic and proliferative potential of the isolated cells. We demonstrated that enrichment based on β1 integrin expression with flow cytometry yields highly clonogenic and proliferative urothelial cells, whereas the rapid adhesion method is not as efficient, possibly because of the unique nature of urothelium, a transitional epithelium, compared to results reported in stratified and columnar epithelia.
AB - With a lack of distinct stem cell markers, isolation of tissue-specific stem cells for tissue engineering and gene therapy is a great challenge. Beta (β)1 integrin expression has been used as a way of enriching for putative epithelial stem cells through rapid adhesion to collagen IV or flow cytometry. This is a first report of enrichment of putative urothelial stem cells using rapid adhesion and flow cytometric methods. We assessed our success by determining the clonogenic and proliferative potential of the isolated cells. We demonstrated that enrichment based on β1 integrin expression with flow cytometry yields highly clonogenic and proliferative urothelial cells, whereas the rapid adhesion method is not as efficient, possibly because of the unique nature of urothelium, a transitional epithelium, compared to results reported in stratified and columnar epithelia.
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U2 - 10.1089/ten.2006.0132
DO - 10.1089/ten.2006.0132
M3 - Article
VL - 13
SP - 263
EP - 270
JO - Tissue Engineering
JF - Tissue Engineering
SN - 1076-3279
IS - 2
ER -