β7-integrin-independent enhancement of mucosal and systemic anti-HIV antibody responses following combined mucosal and systemic gene delivery

Amanda Goodsell, Fengmin Zhou, Soumi Gupta, Manmohan Singh, Padma Malyala, Jina Kazzaz, Catherine Greer, Harold Legg, Tony Tang, January Zur Megede, Ranjana Srivastava, Susan W. Barnett, John J. Donnelly, Paul A Luciw, John Polo, Derek T. O'Hagan, Michael Vajdy

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Vaccination strategies that can block or limit heterosexual human immunodeficiency virus (HIV) transmissions to local and systemic tissues are the goal of much research effort. Herein, in a mouse model, we aimed to determine whether the enhancement of antibody responses through mucosal and systemic immunizations, previously observed with protein-based vaccines, applies to immunizations with DNA- or RNA-based vectors. Intranasal (i.n.) followed by intramuscular (i.m.) immunizations (i.n./i.m.) with polylactide-coglycolide (PLG)-DNA microparticles encoding HIV-gag (PLG-DNA-gag) significantly enhanced serum antibody responses, compared with i.m., i.n. or i.m. followed by i.n. (i.m./i.n.) immunizations. Moreover, while i.n./i.m., i.n. or i.m./i.n. immunizations with PLG-DNA-gag resulted in genital tract antibody responses, i.m. immunizations alone failed to do so. Importantly, β7-deficient mice developed local and systemic antibody responses following i.n./i.m. immunization, or immunization via any other route, similar to those of wild-type mice. To compare the DNA with an RNA delivery system, immunizations were performed with VEE/SIN-gag replicon particles, composed of Venezuelan equine encephalitis virus (VEE) replicon RNA and Sindbis surface structure (SIN). i.n./i.m., compared with any other immunizations, i.n./i.m. immunization with VEE/SIN-gag resulted in enhanced genital tract but not serum antibody responses. These data show for the first time that mucosal followed by systemic immunizations with gene delivery systems enhance B-cell responses independent of the mucosal homing receptors α4β7 and αEβ7.

Original languageEnglish (US)
Pages (from-to)378-389
Number of pages12
JournalImmunology
Volume123
Issue number3
DOIs
StatePublished - Mar 2008

Keywords

  • DNA
  • HIV
  • Migration
  • Mucosal vaccine
  • Reproductive immunology

ASJC Scopus subject areas

  • Immunology

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    Goodsell, A., Zhou, F., Gupta, S., Singh, M., Malyala, P., Kazzaz, J., Greer, C., Legg, H., Tang, T., Zur Megede, J., Srivastava, R., Barnett, S. W., Donnelly, J. J., Luciw, P. A., Polo, J., O'Hagan, D. T., & Vajdy, M. (2008). β7-integrin-independent enhancement of mucosal and systemic anti-HIV antibody responses following combined mucosal and systemic gene delivery. Immunology, 123(3), 378-389. https://doi.org/10.1111/j.1365-2567.2007.02702.x