Abstract
Dermal fibroblasts actively contribute to wound healing by migrating to the wound, synthesizing extracellular matrices, and generating mechanical forces within the wound to initiate wound contraction. Fibroblast-seeded collagen gels provide an in vitro model to study wound contraction. The authors are evaluating the role of the adrenergic signaling system in cutaneous wound repair and recently found that β2-adrenergic receptor (β2-AR) activation markedly decreases keratinocyte migration, an essential step in wound reepithelialization. Because the β2-ARs are also expressed on dermal fibroblasts, a study was initiated to determine the effects of β-adrenergic agonists on dermal fibroblast-mediated collagen gel contraction. A β-agonist (isoproterenol) delayed gel contraction in a dose-dependent manner. A β2-AR specific antagonist (ICI 118,551) prevented the delay, indicating that the β2-AR alone mediated the delay. The active cyclic adenosine monophosphate (cAMP) analog also delayed collagen gel contraction, whereas an inactive cAMP analog partially prevented the delay, suggesting that the mechanism for β-AR agonist-mediated delay was partly cAMP-dependent. Identifying and characterizing agents that modulate wound contraction improves understanding of the wound healing process and could result in novel therapeutic strategies for preventing unwanted wound contraction in burn and trauma patients.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 405-411 |
| Number of pages | 7 |
| Journal | Wound Repair and Regeneration |
| Volume | 13 |
| Issue number | 4 |
| DOIs | |
| State | Published - Jul 2005 |
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ASJC Scopus subject areas
- Dermatology
- Surgery
Cite this
β2-adrenergic receptor activation delays dermal fibroblast-mediated contraction of collagen gels via a cAMP-dependent mechanism. / Pullar, Christine E.; Isseroff, Roslyn Rivkah.
In: Wound Repair and Regeneration, Vol. 13, No. 4, 07.2005, p. 405-411.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - β2-adrenergic receptor activation delays dermal fibroblast-mediated contraction of collagen gels via a cAMP-dependent mechanism
AU - Pullar, Christine E.
AU - Isseroff, Roslyn Rivkah
PY - 2005/7
Y1 - 2005/7
N2 - Dermal fibroblasts actively contribute to wound healing by migrating to the wound, synthesizing extracellular matrices, and generating mechanical forces within the wound to initiate wound contraction. Fibroblast-seeded collagen gels provide an in vitro model to study wound contraction. The authors are evaluating the role of the adrenergic signaling system in cutaneous wound repair and recently found that β2-adrenergic receptor (β2-AR) activation markedly decreases keratinocyte migration, an essential step in wound reepithelialization. Because the β2-ARs are also expressed on dermal fibroblasts, a study was initiated to determine the effects of β-adrenergic agonists on dermal fibroblast-mediated collagen gel contraction. A β-agonist (isoproterenol) delayed gel contraction in a dose-dependent manner. A β2-AR specific antagonist (ICI 118,551) prevented the delay, indicating that the β2-AR alone mediated the delay. The active cyclic adenosine monophosphate (cAMP) analog also delayed collagen gel contraction, whereas an inactive cAMP analog partially prevented the delay, suggesting that the mechanism for β-AR agonist-mediated delay was partly cAMP-dependent. Identifying and characterizing agents that modulate wound contraction improves understanding of the wound healing process and could result in novel therapeutic strategies for preventing unwanted wound contraction in burn and trauma patients.
AB - Dermal fibroblasts actively contribute to wound healing by migrating to the wound, synthesizing extracellular matrices, and generating mechanical forces within the wound to initiate wound contraction. Fibroblast-seeded collagen gels provide an in vitro model to study wound contraction. The authors are evaluating the role of the adrenergic signaling system in cutaneous wound repair and recently found that β2-adrenergic receptor (β2-AR) activation markedly decreases keratinocyte migration, an essential step in wound reepithelialization. Because the β2-ARs are also expressed on dermal fibroblasts, a study was initiated to determine the effects of β-adrenergic agonists on dermal fibroblast-mediated collagen gel contraction. A β-agonist (isoproterenol) delayed gel contraction in a dose-dependent manner. A β2-AR specific antagonist (ICI 118,551) prevented the delay, indicating that the β2-AR alone mediated the delay. The active cyclic adenosine monophosphate (cAMP) analog also delayed collagen gel contraction, whereas an inactive cAMP analog partially prevented the delay, suggesting that the mechanism for β-AR agonist-mediated delay was partly cAMP-dependent. Identifying and characterizing agents that modulate wound contraction improves understanding of the wound healing process and could result in novel therapeutic strategies for preventing unwanted wound contraction in burn and trauma patients.
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U2 - 10.1111/j.1067-1927.2005.130408.x
DO - 10.1111/j.1067-1927.2005.130408.x
M3 - Article
C2 - 16008730
AN - SCOPUS:23044513107
VL - 13
SP - 405
EP - 411
JO - Wound Repair and Regeneration
JF - Wound Repair and Regeneration
SN - 1067-1927
IS - 4
ER -