β-Glucosidase Discovery and Design for the Degradation of Oleuropein

Kathryn G. Guggenheim, Lauren M. Crawford, Francesca Paradisi, Selina C. Wang, Justin Siegel

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Current lye processing for debittering California black table olives produces large amounts of caustic wastewater and destroys many of the beneficial phenolic compounds in the fruit. Herein, we propose using enzyme treatment in place of lye, potentially reducing the amount and causticity of wastewater produced. By specifically targeting the bitterness-causing compound, oleuropein, retention of other beneficial phenolics may be possible. A β-glucosidase from Streptomyces sp. was identified from a screen of 22 glycosyl hydrolases to completely degrade oleuropein in 24 h. Computational modeling was performed on this enzyme, and mutation C181A was found to improve the rate of catalysis by 3.2-fold. This mutant was tested in the context of the olive fruit and leaf extract. Degradation was observed in the olive leaf extract but not in the fruit matrix, suggesting that enzyme fruit penetration is a limiting factor. This work discovers and begins the refinement process for an enzyme that has the catalytic properties for debittering olives and provides direction for future engineering efforts required to make a product with commercial value.

Original languageEnglish (US)
Pages (from-to)15754-15762
Number of pages9
JournalACS Omega
Volume3
Issue number11
DOIs
StatePublished - Nov 20 2018
Externally publishedYes

Fingerprint

Glucosidases
Fruits
Lye
Enzymes
Caustic soda
Degradation
Wastewater
Hydrolases
Caustics
Alkalinity
Catalysis
oleuropein
Processing

ASJC Scopus subject areas

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Guggenheim, K. G., Crawford, L. M., Paradisi, F., Wang, S. C., & Siegel, J. (2018). β-Glucosidase Discovery and Design for the Degradation of Oleuropein. ACS Omega, 3(11), 15754-15762. https://doi.org/10.1021/acsomega.8b02169

β-Glucosidase Discovery and Design for the Degradation of Oleuropein. / Guggenheim, Kathryn G.; Crawford, Lauren M.; Paradisi, Francesca; Wang, Selina C.; Siegel, Justin.

In: ACS Omega, Vol. 3, No. 11, 20.11.2018, p. 15754-15762.

Research output: Contribution to journalArticle

Guggenheim, KG, Crawford, LM, Paradisi, F, Wang, SC & Siegel, J 2018, 'β-Glucosidase Discovery and Design for the Degradation of Oleuropein', ACS Omega, vol. 3, no. 11, pp. 15754-15762. https://doi.org/10.1021/acsomega.8b02169
Guggenheim, Kathryn G. ; Crawford, Lauren M. ; Paradisi, Francesca ; Wang, Selina C. ; Siegel, Justin. / β-Glucosidase Discovery and Design for the Degradation of Oleuropein. In: ACS Omega. 2018 ; Vol. 3, No. 11. pp. 15754-15762.
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