β-Carotene inhibits the oxidative modification of low-density lipoprotein

Ishwarlal Jialal, Edward P. Norkus, Louis Cristol, Scott M. Grundy

Research output: Contribution to journalArticle

212 Citations (Scopus)

Abstract

Several lines of evidence indicate that oxidized LDL (Ox-LDL) may promote atherogenesis. Hence, the role of antioxidants in the prevention of LDL oxidation needs to be determined. β-Carotene, in addition to being an efficient quencher of singlet oxygen, can also function as a radical-trapping antioxidant. Since previous studies have failed to show that β-carotene inhibits LDL oxidation, we re-examined its effect on the oxidative modification of LDL. For these studies, LDL was oxidized in both a cell-free (2.5 μM Cu2+ in PBS) and a cellular system (human monocyte macrophages in Ham's F-10 medium). β-Carotene inhibited the oxidative modification of LDL in both systems as evidenced by a decrease in the lipid peroxide content (thiobarbituric-acid-reacting substances activity), the negative charge of LDL (electrophoretic mobility) and the formation of conjugated dienes. By inhibiting LDL oxidation, β-carotene substantially decreased its degradation by macrophages. β-Carotene (2 μM) was more potent than α-tocopherol (40 μM) in inhibiting LDL oxidation. Thus, β-carotene, like ascorbate and α-tocopherol, inhibits LDL oxidation and might have an important role in the prevention of atherosclerosis.

Original languageEnglish (US)
Pages (from-to)134-138
Number of pages5
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume1086
Issue number1
DOIs
StatePublished - Oct 15 1991
Externally publishedYes

Fingerprint

Carotenoids
LDL Lipoproteins
Oxidation
Tocopherols
Macrophages
Atherosclerosis
Antioxidants
Singlet Oxygen
Lipid Peroxides
oxidized low density lipoprotein
Electrophoretic mobility
Monocytes
Degradation
low density lipoprotein inhibitor

Keywords

  • (Human macrophage)
  • Antioxidant
  • Atherosclerosis
  • Lipoprotein
  • β-Carotene

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Endocrinology

Cite this

β-Carotene inhibits the oxidative modification of low-density lipoprotein. / Jialal, Ishwarlal; Norkus, Edward P.; Cristol, Louis; Grundy, Scott M.

In: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism, Vol. 1086, No. 1, 15.10.1991, p. 134-138.

Research output: Contribution to journalArticle

Jialal, Ishwarlal ; Norkus, Edward P. ; Cristol, Louis ; Grundy, Scott M. / β-Carotene inhibits the oxidative modification of low-density lipoprotein. In: Biochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism. 1991 ; Vol. 1086, No. 1. pp. 134-138.
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