α3β1 integrin modulates neuronal migration and placement during early stages of cerebral cortical development

Ralf S. Schmid, Stephanie Crossen, Amelia Stanco, Yukako Yokota, Jordan A. Kreidberg, E. S. Anton

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

We show that α3 integrin mutation disrupts distinct aspects of neuronal migration and placement in the cerebral cortex. The preplate develops normally in α3 integrin mutant mice. However, time lapse imaging of migrating neurons in embryonic cortical slices indicates retarded radial and tangential migration of neurons, but not ventricular zone-directed migration. Examination of the actin cytoskeleton of α3 integrin mutant cortical cells reveals aberrant actin cytoskeletal dynamics at the leading edges. Deficits are also evident in the ability of developing neurons to probe their cellular environment with filopodial and lamellipodial activity. Calbindin or calretinin positive upper layer neurons as well as the deep layer neurons of α3 integrin mutant mice expressing EGFP were misplaced. These results suggest that α3β1 integrin deficiency impairs distinct patterns of neuronal migration and placement through dysregulated actin dynamics and defective ability to search and respond to migration modulating cues in the developing cortex.

Original languageEnglish (US)
Pages (from-to)6023-6031
Number of pages9
JournalDevelopment
Volume131
Issue number24
DOIs
StatePublished - Dec 1 2004
Externally publishedYes

Fingerprint

Integrins
Neurons
Aptitude
Actins
Time-Lapse Imaging
Calbindin 2
Calbindins
Actin Cytoskeleton
Cerebral Cortex
Cues
Mutation

Keywords

  • Adhesion
  • Cerebral cortex
  • Migration

ASJC Scopus subject areas

  • Anatomy
  • Cell Biology

Cite this

α3β1 integrin modulates neuronal migration and placement during early stages of cerebral cortical development. / Schmid, Ralf S.; Crossen, Stephanie; Stanco, Amelia; Yokota, Yukako; Kreidberg, Jordan A.; Anton, E. S.

In: Development, Vol. 131, No. 24, 01.12.2004, p. 6023-6031.

Research output: Contribution to journalArticle

Schmid, Ralf S. ; Crossen, Stephanie ; Stanco, Amelia ; Yokota, Yukako ; Kreidberg, Jordan A. ; Anton, E. S. / α3β1 integrin modulates neuronal migration and placement during early stages of cerebral cortical development. In: Development. 2004 ; Vol. 131, No. 24. pp. 6023-6031.
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