α-particle energy loss measurement of microgram depositions of biomolecules

Patrick G. Grant, Magnus Palmblad, Steven Murov, Darren J. Hillegonds, Dawn L. Ueda, John S. Vogel, Graham Bench

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

A commercially available α-particle spectrometer and 210Po α-particle source were used to determine the mass of microgram quantities of biomolecules. Samples were deposited in microliter volumes on thin silicon nitride windows and dried. The energy loss of the α-particles after traversing the sample was converted to a mass using tabulated α-particle stopping powers. The measurement was absolute, independent of biomolecule species, and no standards were needed for quantitation. The method has a dynamic range of 0.1-100 μg for deposits of diameter 1-2 mm. The precision varies from ∼20% at 100 ng to a few percent at 5-100 μg. The silicon nitride windows allow multimodal analysis of the same quantified sample, including PIXE probing of elemental abundances, molecular identification by mass spectrometry, and isotopic quantitation of interactions. The method was used with accelerator mass spectrometry to quantify specific activities of microgram quantities of 14C-labeled proteins.

Original languageEnglish (US)
Pages (from-to)4519-4524
Number of pages6
JournalAnalytical Chemistry
Volume75
Issue number17
DOIs
StatePublished - Sep 1 2003
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry

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    Grant, P. G., Palmblad, M., Murov, S., Hillegonds, D. J., Ueda, D. L., Vogel, J. S., & Bench, G. (2003). α-particle energy loss measurement of microgram depositions of biomolecules. Analytical Chemistry, 75(17), 4519-4524. https://doi.org/10.1021/ac034170g