α-Gal oligosaccharides: Chemistry and potential biomedical application

A. Janczuk, J. Li, W. Zhang, Xi Chen, Y. Chen, J. Fang, J. Wang, P. G. Wang

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

This article focuses on the most recent research efforts by the Wang group in the field of α-Gal oligosaccharides, α-Gal oligosaccharides are carbohydrate structures bearing a Galα1-3Galβ terminus. This class of compounds are believed to act as xenoactive antigens that instigate the hyperacute rejection in xenotransplantation. Enzymatic methods using recombinant α1-3 galactosyltransferase were employed to synthesize several α-Gal oligosaccharides. In addition, a chemical synthetic scheme was devised in order to produce readily acessible amounts of α-Gal. Conformational analysis was done using both NMR techniques and molecular modeling protocols. These studies provide important information in the structure-function relationship of α-Gal and anti-α-Gal antibodies. The second part of this article deals with the use of α-Gal in immunotherapy. Based on the abundance of anti-α-Gal antibodies IgM and IgG in human blood serum, α-Gal conjugates may act as effective immunotherapeutic drugs. Recent studies have discovered characteristic peptide sequences containing Arg-Gly-Asp (RGD) motif that binds to integrins on the surface of cancer cells. A α-Gal conjugate was developed by chemical coupling a α-Gal trisaccharide to a RGD peptide. Preliminary studies using the conjugate on prostate DU-145 cancer cells showed a reduction in the survival rate of the cells.

Original languageEnglish (US)
Pages (from-to)155-164
Number of pages10
JournalCurrent Medicinal Chemistry
Volume6
Issue number2
StatePublished - 1999
Externally publishedYes

ASJC Scopus subject areas

  • Organic Chemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Pharmacology

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  • Cite this

    Janczuk, A., Li, J., Zhang, W., Chen, X., Chen, Y., Fang, J., Wang, J., & Wang, P. G. (1999). α-Gal oligosaccharides: Chemistry and potential biomedical application. Current Medicinal Chemistry, 6(2), 155-164.