α-catenin is a molecular switch that binds E-cadherin-β-catenin and regulates actin-filament assembly

Frauke Drees, Sabine Pokutta, Soichiro Yamada, W. James Nelson, William I. Weis

Research output: Contribution to journalArticle

682 Citations (Scopus)

Abstract

Epithelial cell-cell junctions, organized by adhesion proteins and the underlying actin cytoskeleton, are considered to be stable structures maintaining the structural integrity of tissues. Contrary to the idea that α-catenin links the adhesion protein E-cadherin through β-catenin to the actin cytoskeleton, in the accompanying paper we report that α-catenin does not bind simultaneously to both E-cadherin-β-catenin and actin filaments. Here we demonstrate that α-catenin exists as a monomer or a homodimer with different binding properties. Monomeric α-catenin binds more strongly to E-cadherin-β-catenin, whereas the dimer preferentially binds actin filaments. Different molecular conformations are associated with these different binding states, indicating that α-catenin is an allosteric protein. Significantly, α-catenin directly regulates actin-filament organization by suppressing Arp2/3-mediated actin polymerization, likely by competing with the Arp2/3 complex for binding to actin filaments. These results indicate a new role for α-catenin in local regulation of actin assembly and organization at sites of cadherin-mediated cell-cell adhesion.

Original languageEnglish (US)
Pages (from-to)903-915
Number of pages13
JournalCell
Volume123
Issue number5
DOIs
StatePublished - Dec 2 2005
Externally publishedYes

Fingerprint

Catenins
Cadherins
Actin Cytoskeleton
Actins
Switches
Adhesion
Actin-Related Protein 2-3 Complex
Molecular Conformation
Proteins
Intercellular Junctions
Cell adhesion
Structural integrity
Cell Adhesion
Polymerization
Dimers
Conformations
Monomers
Epithelial Cells
Tissue

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology

Cite this

α-catenin is a molecular switch that binds E-cadherin-β-catenin and regulates actin-filament assembly. / Drees, Frauke; Pokutta, Sabine; Yamada, Soichiro; Nelson, W. James; Weis, William I.

In: Cell, Vol. 123, No. 5, 02.12.2005, p. 903-915.

Research output: Contribution to journalArticle

Drees, Frauke ; Pokutta, Sabine ; Yamada, Soichiro ; Nelson, W. James ; Weis, William I. / α-catenin is a molecular switch that binds E-cadherin-β-catenin and regulates actin-filament assembly. In: Cell. 2005 ; Vol. 123, No. 5. pp. 903-915.
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