α- and γ-tocopherol prevent age-related transcriptional alterations in the heart and brain of mice

We used high-density oligonucleotide arrays to measure transcriptional alterations in the heart and brain (neocortex) of 30-mo-old B6C3F₁ mice supplemented with α-tocopherol (αT) and γ-tocopherol (γT) since middle age (15 mo). Gene expression profiles were obtained from 5- and 30-mo-old control mice and 30-mo-old mice supplemented with αT (1 g/kg) or a mixture of αT and γT (500 mg/kg of each tocopherol) from middle age (15 mo). In the heart, both tocopherol-supplemented diets were effective in inhibiting the expression of genes previously associated with cardiomyocyte hypertrophy and increased innate immunity. In the brain, induction of genes encoding ribosomal proteins and proteins involved in ATP biosynthesis was observed with aging and was markedly prevented by the mixture of αT and γT supplementation but not by αT alone. These resultsdemonstrate that middle age-onset dietary supplementation with αT and γT can partially prevent age-associated transcriptional changes and that these effects are tissue and tocopherol specific.