Targeted- and timed-releasing nanotherapeutics against leukemia stem cells

Project: Research project

Description

? DESCRIPTION (provided by applicant): The goal of this project is to develop nanotherapeutics that can specifically deliver the chemotherapeutic drug daunorubicin into acute myeloid leukemia (AML) stem cells (targeted delivery) and release the drug inside the cells (timed release) in order to eradicate this cell population. Current chemotherapy for AML is disappointing in that it can cure only a minority of AML patients and is associated with severe toxicity and significant mortality even with intensive inpatient monitoring and supportive care. Leukemia stem cells (LSC) are relatively resistant to the conventional chemotherapy and can subsequently produce more leukemia cells to cause disease recurrence. In order to cure leukemia, LSC must be eradicated. The C-type lectin-like molecule-1 (CLL-1) is a cell surface protein that is specifically expressed on most AML LSC, but not on normal hematopoietic stem cells. We have developed a peptide that specifically targets CLL1. When this peptide is covalently attached to a nanometer-scale micellar drug formulation, the resulted targeting nanoparticles, called micelles, can specifically deliver the drug load into cells expressing CLL1, including clinical leukemia specimens. The drug-loaded, CLL1-targeting micelles are stable in blood, and can potentially improve therapy against AML by (1) delivering a high local concentration of daunorubicin specifically into LSC, overwhelming the resistant mechanisms and eradicating LSC; (2) killing leukemic cells throughout the body with the release of daunorubicin from the micelles and LSC into circulation; (3) decreasing therapy-induced toxicity and mortality owing to the sequestration of the drug inside the micelles; and (4) allowing administration of high-dose daunorubicin with reduced toxicity through formulation of daunorubicin into micelles.
StatusActive
Effective start/end date4/15/153/31/20

Funding

  • National Institutes of Health: $358,851.00
  • National Institutes of Health: $357,422.00

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Leukemia
Stem Cells
Daunorubicin
Micelles
Acute Myeloid Leukemia
Pharmaceutical Preparations
C-Type Lectins
Myeloid Progenitor Cells
Drug Therapy
Drug Compounding
Peptides
Mortality
Hematopoietic Stem Cells
Nanoparticles
Inpatients
Membrane Proteins
Recurrence
Therapeutics
Population

ASJC

  • Medicine(all)