SOUTHWESTERN REGIONAL IVEM AND IMAGE ANALYSIS RESOURCE

  • Fomina, Alla F (PI)
  • Milligan, Ronald (PI)
  • Thompson, Wesley (PI)
  • Covell, James (PI)
  • Doxsey, Steven (PI)
  • Groves, Philip (PI)
  • Tang, Hengli (PI)
  • Murakami, Fujio (PI)
  • Ouyang, Yannan (PI)
  • Traynor-Kaplan, Alexis (PI)
  • Citovsky, Vitaly (PI)
  • Levinson, Simon (PI)
  • Hama, Kyoshi (PI)
  • Mironov, Alexander (PI)
  • Roberts, William (PI)
  • Schikorski, Thomas (PI)
  • Salisbury, Jeffrey (PI)
  • Jeromin, Andreas (PI)
  • Lyden, P.A.T. (PI)
  • Allen, Gethyn (PI)
  • Callaway, E.D. (PI)
  • Salomon, Christine (PI)
  • Stokes, David (PI)
  • Brown, Joan Heller (PI)
  • Schultz, David (PI)
  • Luther, Paul (PI)
  • Brodin, Lennart (PI)
  • Barak, Yaacov (PI)
  • Berg, Darwin (PI)
  • Moisan, Tiffany (PI)
  • Huang, S.U.I. (PI)
  • Sanna, Pietro (PI)
  • Halpain, Shelley (PI)
  • Sheikh, Kazim (PI)
  • Witztum, Joseph (PI)
  • Bridgman, Paul (PI)
  • Martins-Green, Manuella (PI)
  • Ikeda, Yasohiro (PI)
  • Mercier, Frederic (PI)
  • McDonough, Patrick (PI)
  • Stuiver, Ingrid (PI)
  • Knowlton, Kirk (PI)
  • Dennis, Edward (PI)
  • Gaietta, Guido (PI)
  • Woodcock, Christopher L. (PI)
  • Schwartz, Eric (PI)
  • Pourquie, Olivier (PI)
  • Palisano, John (PI)
  • Aman, M. Javad (PI)
  • Ong, Albert (PI)
  • Benaroch, Philippe (PI)
  • Olson, Arthur J. (PI)
  • Pugnaloni, Amanda (PI)
  • Akassoglou, Katerina (PI)
  • Schultz, Sheldon (PI)
  • Saier, Milton H. (PI)
  • Fredrickson, James (PI)
  • Hampton, Randolph Y. (PI)
  • Albani, Salvatore (PI)
  • Mancini, Michael (PI)
  • Weiss, James (PI)
  • Salomon, Daniel R. (PI)
  • Holt, Christine (PI)
  • Keller, Steve (PI)
  • Takaoka, Akio (PI)
  • Bier, Ethan (PI)
  • Fine, Alan Fine (PI)
  • Huang, S.U.I. (PI)
  • Horwitz, Alan (PI)
  • Caplan, Michael J. (PI)
  • Karin, Michael (PI)
  • Storrie, Brian (PI)
  • Shupliakov, Oleg (PI)
  • Cirulli, Vincenzino (PI)
  • Koster, B. (PI)
  • Johnson, Ross (PI)
  • Sanna, Pietro (PI)
  • Neel, Benjamin G. (PI)
  • Heckman, Carol (PI)
  • Waxham, M. Neal (PI)
  • Haydon, Philip (PI)
  • Chao, Moses Victor (PI)
  • Turner, Eric E. (PI)
  • Karpen, Gary H. (PI)
  • Zipursky, S. (PI)
  • Toga, Arthur (PI)
  • Thali, Markus (PI)
  • Insel, Paul (PI)
  • Malenka, Robert (PI)
  • Fraser, Ian (PI)
  • Caldwell, Jonathan (PI)
  • Schad, Gerhard (PI)
  • Duncan, Rory (PI)
  • Rice, Charles M. (PI)
  • Kennedy, Mary (PI)
  • Tsien, Richard (PI)
  • Laird, Dale (PI)
  • Moisan, Tiffany (PI)
  • Smalheiser, Neil (PI)
  • Green, Douglas (PI)
  • Wilson, Bridget (PI)
  • Francis, Howard (PI)
  • Cleveland, Don W. (PI)
  • Nguyen, Xuong (PI)
  • Pekny, Milos (PI)
  • Frey, Terrence (PI)
  • Howell, Stephen (PI)
  • McCulloch, Andrew D. (PI)
  • Ahlquist, Paul G. (PI)
  • Chen, J.U. (PI)
  • Sejnowski, Terrence (PI)
  • Greenough, William (PI)
  • Tebo, Bradley M. (PI)
  • Gleeson, Joseph (PI)
  • Taylor, Susan (PI)
  • Taylor, Palmer William (PI)
  • Masliah, Eliezer (PI)
  • Spector, Deborah (PI)
  • Lohse, Martin (PI)
  • Scheffler, Immo (PI)
  • Volkmann, Niels (PI)
  • Hedrick, Stephen (PI)
  • Nishiyama, Akiko (PI)
  • Bessereau, Jean-Louis (PI)
  • Chien, Kenneth (PI)
  • Sungho, Jin (PI)
  • Dowhan, William (PI)
  • [No Value], Paul N. (PI)
  • Trapp, Bruce (PI)
  • Ribak, Charles (PI)
  • Everts, Maaike (PI)
  • Auer, Manfred (PI)
  • Pakrasi, Himadri (PI)
  • Ross, Robert (PI)
  • Jeromin, Andreas (PI)
  • Dennis, Edward (PI)
  • McCarren, J.A.Y. (PI)
  • Chessler, Steven (PI)
  • Slesinger, Paul (PI)
  • Baldock, Richard (PI)
  • Isaacson, Jeffrey (PI)
  • Wen-Hong, Li (PI)
  • Traver, David (PI)
  • Cahalan, Michael (PI)
  • Fisher, Steven A. (PI)
  • Kitagawa, Katsumi (PI)
  • Santelli, Glaucia (PI)
  • Von Herrath, Matthias G. (PI)
  • Blankenship, Robert (PI)
  • Fung, Yuan-Cheng (PI)
  • Halegoua, Simon (PI)
  • Attias, Marcia (PI)
  • Crawford, Brian (PI)
  • Murre, Cornelis (PI)
  • Gaietta, Guido (PI)
  • Chengzhi, Cai (PI)
  • Daniel, Ju (PI)
  • Bang, Marie-Louise (PI)
  • Schroeder, Julian (PI)
  • Gruenberg, Jean (PI)
  • Leube, Rudolf (PI)
  • Sheikh, Farah (PI)
  • Bandaru, Prabhakar (PI)
  • Hildebrand, Mark (PI)
  • Welsh, John (PI)
  • van Dyke, Terry (PI)
  • Taupenot, Laurent (PI)
  • Williams, David Collin (PI)
  • Lanman, Jason Kenneth (PI)
  • Sung, A.M.Y. (PI)
  • Newton, Alexandra C. (PI)
  • Johnson, Randall (PI)
  • Buck, Martina (PI)
  • Evans, Sylvia (PI)
  • Sah, Robert (PI)
  • Condorelli, Gianluigi (PI)
  • Breen, David Edward (PI)
  • Nemat-Nasser, S.I.A. (PI)
  • Xuong, Nguyen-Huu (PI)
  • Winslow, Raimond Lester (PI)
  • Curiel, David Terry (PI)
  • Ross, Christopher (PI)
  • Cirulli, Vincenzino (PI)
  • Adams, Stephen (PI)
  • Scanziani, Massimo (PI)
  • Suetterlin, Christine (PI)
  • Dixon, Jack (PI)
  • Yamaguchi, Ryuji (PI)
  • Daniel, Ju (PI)
  • Li, J.U.N. (PI)
  • Cline, Hollis (PI)
  • Granneman, James (PI)
  • Malek, Moh H. (PI)
  • Pasquale, Elena (PI)
  • O'Shea, Clodagh (PI)
  • Gage, Fred (PI)
  • Baldwin, J. (PI)
  • Newmeyer, Donald David (PI)
  • Caron, Marc G. (PI)
  • Bingren, Hu (PI)
  • Beyer, Eric (PI)
  • Weinberg, Richard (PI)
  • Sosinsky, Gina Ellen (PI)
  • Johnson, John (PI)
  • Bossy-Wetzel, Ella (PI)
  • Fox, Donald (PI)
  • Spirou, George (PI)
  • Perez, Gloria Ines (PI)
  • Gourdie, Robert (PI)
  • Martone, Maryann (PI)
  • McAdams, Harley Hobbard (PI)
  • Ghosh, Anirvan (PI)
  • Lampe, Paul (PI)
  • Hoshijima, Masahiko (PI)
  • Dauer, William (PI)
  • Akassoglou, Katerina (PI)
  • Callaway, Edward (PI)
  • Yang, Jerry (PI)
  • Schnitzer, Mark (PI)
  • Lindsey, James (PI)
  • Lichtman, Jeff (PI)
  • Hess, Harold (PI)
  • Nakagawa, Terunaga (PI)
  • Shawkey, Matthew (PI)
  • Yishi, Jin (PI)
  • Price, Paul Arms (PI)
  • Kirkland, Angus (PI)
  • Smith, Stephen (PI)
  • Bewersdorf, Joerg (PI)
  • Dillmann, Wolfgang (PI)
  • Karin, Michael (PI)
  • George, Julia (PI)
  • Haddad, Gabriel (PI)
  • Lysakowski, Anna (PI)
  • Johnson, Christopher (PI)
  • Verma, Inder Mohan (PI)
  • Sailor, Michael (PI)
  • Firtel, Richard (PI)
  • Pogliano, Kit (PI)
  • Oland, Lynne (PI)
  • West, John (PI)
  • Guatelli, John C. (PI)
  • De Schutter, Erik (PI)
  • Kornberg, Thomas B. (PI)
  • Clapham, David (PI)
  • Sander, Maike (PI)
  • Hamdoun, Amro (PI)
  • McCarthy, Ken Douglas (PI)
  • Baldwin, Kristin (PI)
  • Printz, Morton (PI)
  • Marsh-Armstrong, Nicholas (PI)
  • Comoletti, Davide (PI)
  • Nicoll, Roger (PI)
  • Fisher, Steven A. (PI)
  • Terrence, I. (PI)
  • Sutko, John (PI)
  • Malecid, Marek (PI)
  • Flossie, It (PI)
  • Belmont, Andrew (PI)
  • Gastinel, Louis (PI)
  • Hofftnan, Harold (PI)
  • Shrager, Peter (PI)
  • Wilson, Charles (PI)
  • Citovsky, Vitaiy (PI)
  • Forer, Arthur (PI)
  • Traynor-Kaplan, Mexis (PI)
  • Carragher, Bridget (PI)
  • Quaranta, Vito (PI)
  • Sultan, Fahad (PI)
  • Kusakabe, Tatsumi (PI)
  • Hanneken, Anne (PI)
  • Doxey, Steve (PI)
  • Kelley, Darcy (PI)
  • Masliah, Eliezer (PI)
  • Hardison, William (PI)
  • Malhotra, Vivek (PI)
  • Kun Ping, Lu (PI)
  • Kosik, K.E.N. (PI)
  • Schachner, Melitta (PI)
  • Boss, Gerry (PI)
  • Greenough, William (PI)
  • Kuchitsu, Kazuyuki (PI)
  • Carey, Lai (PI)
  • Sosinsky, Gina (PI)
  • Spector, David (PI)
  • Jacobs, Gwen (PI)
  • Perkins, Guy (PI)
  • Salomon, Daniel Rene (PI)
  • Cirulli, Vincenzino (PI)
  • Bngren, Hu (PI)
  • Young, Stephen (PI)
  • Gary, Fan (PI)
  • Patrick, Nef (PI)
  • Attias, Marcia (PI)
  • Arbuthnott, Gordon (PI)
  • Guatelli, John (PI)
  • Ellisman, Mark (PI)

Project: Research project

Description

A Regional Resource for computer-aided Intermediate Voltage Transmission
Electron Microscopy (300-400 KV, IVEM) and 3-dimensional (3-D) image
analysis will be established & serve the Southwest region of the country.
These facilities would be
made available immediately to investigators using established techniques
and working on biological problems that could clearly benefit from the
use of thicker specimens and 3-D imaging. We have directed our plan for
core technological research toward the development of technologies that
will contribute to research in structural neurobiology as well as cell
biology and molecular biology. Our goals for collaboration, service,
training and dissemination are all related to expanding the use ot these
technologies so that they can become as valuable as possible for the
biomedical community We efmphasize the use of computer aided methods for
enhancement of image contrast, extraction of accurate 3-dimentional
information from within single thick specimens and reconstruction of
larger structural complexes with serial thich section analysis. We are
planning to use a variety of contrast enhancing methods on model
system/biological problems that will clearly benefit from the unique
capabilities of a computer-coupled IVEM. These include core technological
research activities to: provide new IVEM compatible macromolecular
probes; improve penetration of probes into frozen sections; enhance
selective staining of subcellular structure; and define the 3-D
distributions of and relationships between submicron cell processes in
nervous systems with quantitative accuracy. The capabilities to be
developed and research accomplishments anticipated in all of the core
project areas involving methods of specimen preparation will be augmented
by features of the imaging system to be developed. These will include:
user interactive modification of operating conditions of the IVEM in
order to alter image contrast; microscope-coupled image processing and
analysis; and semi-automated 3-D image acquisition and reconstruction
facilities. Training of users in the application of the technology will
occur at the Resource. In addition, yearly workshops and various other
forms of scientific communication will be used to disseminate the to-be-
developed Resource technologies.
StatusFinished
Effective start/end date12/12/883/31/14

Funding

  • National Institutes of Health

Fingerprint

Interleukin-2
Three-Dimensional Imaging
Technology
T-Lymphocytes
Research
Neurobiology
Frozen Sections
Molecular Biology
Microscopy
Research Personnel
Staining and Labeling
Education

ASJC

  • Medicine(all)