PATHOGENESIS OF MOUSE HEPATITIS VIRUS IN LABORATORY MICE

Project: Research project

Project Details

Description

Mouse hepatitis virus (MHV) is a prevalent, highly contagious coronavirus
with significant impact on the research usefulness of infected mice. The
MHV group, like other coronaviruses, contains strains with primary
respiratory or enterotropism. The overall objective of this project is to
critically examine the pathogenesis of enterotropic MHV, which is the least
studied but most common form of Information gained from these studies will
have direct practical relevance to accurate diagnosis, control and
management of MHV in laboratory mouse populations. Specific aims of this
project include examination of host age and genotype related differentials
in susceptibility, duration and response to infection; the role of
intestinal mucosal proliferative kinetics in host susceptibility and
response; mechanisms of dissemination of virus from intestine to other
organs; maternally-derived passive immunity to infection; and virus strain
specificity of host challenge immunity. These studies will involve
inoculation (:)f BALB/cByJ, SJL/J and other mouse genotypes wit'-. MHV at 2
days and 1,2,3 or 12 weeks of age. Host responses will be examined at
intervals after infection. Methodology to assess infection will include
histology, MHV immunohistochemistry, mucosal proliferative kinetics
(including autoradiography), infectious virus detection and quantification,
viral nucleic acid hybridization and serology (IgA, IgG and IgM responses).
Data generated from these studies will not only be relevant to laboratory
mice, but will also serve to define MHV as a useful general model of
coronaviral enteritis, a widespread and important cause of neonatal disease
in man and animals.
StatusFinished
Effective start/end date1/1/848/31/99

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $204,758.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

ASJC

  • Medicine(all)

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