PATHOGENESIS OF MEMBRANOUS GLOMERULONEPHROPATHY

Project: Research project

Description

The objective of this research proposal is to understand more about the
pathogeneses of the autologous immune-complex nephropathy of rats (AIC) and of
the idiopathic membranous glomerulonephropathy (MGN) in humans. Specifically:
1) we will test the hypothesis that the susceptibility of a species of an animal
to develop AIC or of a human to develop MGN is related to the excretion of a
brush border antigen (BBAg) in the urine of that species or the individual, 2)
develop a more specific radioimmunoassay for measuring BBAg and study in depth
the pathogenesis of AIC, 3) determine the ultrastructural location of BBAg in
kidney, 4) determine if BBAg is produced by the cultured proximal renal tubules,
5) study the effect of an artifically produced physical-barrier in the
glomerular basement membrane induced by the oral administration of silver
nitrate on the immune-complex localization in the subepithelial position and 6)
study the effect of exogenously given antigen excess as a therapeutic maneuver
on the course of AIC. The methods will include immunofluorescence, horseradish
peroxidase labelled specific antibodies to BBAg and electron microscopy,
culturing of proximal renal tubular cells, radioimmunoassay and column
chromatography, etc. Data collected from these experiments will: 1) enhance
our knowledge about the pathogenesis of AIC of rats and possibly of MGN in
humans, 2) may provide methods to identify individuals susceptible to develop
MGN, 3) may lead to methods to treat AIC and possibly MGN and 4) produce
information on the role of a physical barrier on the immune-complex deposition
in glomerulonephritis.
StatusFinished
Effective start/end date9/1/838/31/87

Funding

  • National Institutes of Health

Fingerprint

Membranous Glomerulonephritis
Antigen-Antibody Complex
Architectural Accessibility
Radioimmunoassay
Antigens
Proximal Kidney Tubule
Glomerulonephritis
Basement Membrane
Fluorescent Antibody Technique
Oral Administration
Electron Microscopy
Research Design
Urine
Kidney
Antibodies
Therapeutics

ASJC

  • Medicine(all)