OXYGENATION IN VIVO WITH 1H NMR MYOGLOBIN SIGN

Project: Research project

Description

Oxygen is a fundamental regulator of cellular bioenergetics and
intermediary metabolism. Observing tissue oxygen level, however, has
faced many hurdles. Extant techniques confront major obstacles in
sampling intracellular oxygenation in a localized tissue region.
Consequently a key question cannot be posed: Is the accepted view of the
interaction between oxygen level and the regulation of bioenergetics,
derived from model studies, also accurate in vivo? NMR offers a unique opportunity to answer such questions in vivo. Our
preliminary data have demonstrated the 1H NMR visibility of the myoglobin
proximal histidyl F8 NH and Val Ell methyl groups in heart muscle. These
signals directly reflect intracellular oxygenation, in contrast to the
commonly used 31P peaks, which monitor only the cellular interaction. We propose then to establish firmly the 1H NMR techniques to observe
oxygen tension in specific tissue regions in vivo. Clearly the capacity
to measure tissue oxygen opens many applications with both physiological
and pathophysiological perspectives.
StatusFinished
Effective start/end date2/1/921/31/98

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

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Myoglobin
Oxygen
Energy Metabolism
Myocardium
Proton Magnetic Resonance Spectroscopy

ASJC

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)