ONCOGENE AND ACTIVATOR: TUMORIGENESIS BY CLONED DNA

Project: Research project

Description

The studies of the action and activation of viral and cellular oncogenes
represent a major focus in the current cancer research. Several approaches
relying on in vivo virus infection or in vitro DNA transfection have been
established; each has its own advantages and disadvantages. Recently we
have developed a procedure whereby we can inject cloned DNA fragments
directly into the wing-web of chickens and assess the in vivo sarcomagenic
ability of the cloned DNA. We found that isolated v-src, the oncogene
carried by Rous sarcoma virus can efficiently induce tumor nodules at the
injection site, within two to three weeks. All the tumors analyzed have
acquired v-src DNA in their genomes and express high level of v-src
sequences. This conclusively shows that v-src alone without the help of
other viral genes or viral infections, can trigger the sarcomagenic
process. Interestingly, this sarcomagenic ability of v-src does not
require the presence of a 5' promoter or LTR, but relies on downstream
flanking sequences encompassing the enhancer region. We wish to
substantiate these findings and extend our analysis to study the action and
activation of other viral and cellular oncogenes. In particular, we
describe detailed plans for studying the regulatory element involved in
v-src activation and the oncogenic properties of c-src and c-fps/fes. Our
approach, when fully developed, should not only open a new avenue for
testing the sarcomagenic ability of isolated oncogenes but also provide
means to study their regulations and mode of activations.
StatusFinished
Effective start/end date1/1/8512/31/87

Funding

  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health

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Oncogenes
Carcinogenesis
DNA
Virus Diseases
Virus Activation
Rous sarcoma virus
Neoplasms
Viral Genes
Transfection
Chickens
Genome
Research

ASJC

  • Medicine(all)