ONCOGENE ACTIVATION IN AVIAN B &T LYMPHOMA

Project: Research project

Description

Oncogene activation by retroviral DNA insertion has been
implicated in a variety of neoplasms. The present application
represents our continued effort to understand the molecular
mechanisms involved in the induction of chicken B and T-
lymphomas. In the past two years, we have focused on the
characterization of a novel avian T-lymphoma identified by the
CoPI. This lymphoma is induced by avian reticuloendotheliosis
virus (REV) and resembles Marek's disease herpes virus (MDV)
induced T-lymphoma. We have demonstrated that insertional
activation of c-myc is involved in this disease, but the c-myc
activation pattern differs from that found in B-lymphomas
induced by the same virus. We have also characterized in detail
the structure and expression of a provirus involved in c-myc
activation in one B-lymphoma sample. Our studies, together with
others, have revealed a number of interesting ways that
retroviruses can modulate the LTR promoter activities and their
interactions with host oncogenes. Understanding transcriptional
modulation of viral LTR's and adjacent oncogenes are not only key
to our understanding of the oncogene-activation process but also
important in our appreciation of the complex eucaryotic
transcriptional machinery. This represents a major focus of the
present application. In addition, we wish to continue our
investigation of the possible relationship between REV (retroviral)
and MDV (herpes viral) induced T-lymphomas. In particular, we
propose to further explore the structure and the significance of
the REV LTR-related sequence we have recently identified in the
pathogenic MDV genome. Finally, we propose to investigate the
novel oncogene involved in rare B-lymphomas induced by avian
retroviruses carrying endogenous LTR's.
StatusFinished
Effective start/end date3/1/888/31/02

Funding

  • National Institutes of Health: $184,647.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $320,502.00
  • National Institutes of Health
  • National Institutes of Health: $170,716.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $292,100.00
  • National Institutes of Health
  • National Institutes of Health: $257,688.00
  • National Institutes of Health

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Oncogenes
Reticuloendotheliosis virus
Lymphoma
Herpesviridae
Retroviridae
Marek Disease
Insertional Mutagenesis
RNA
Avian Reticuloendotheliosis
B-Lymphocytes
Carcinogenesis
Genes
T-Lymphocytes
Avian Leukosis Virus
Viruses
Cancer Vaccines
Retroviridae Infections
Genome
Proviruses
Molecular Biology

ASJC

  • Medicine(all)