OLIGODENDROGLIAL PROLIFERATION AND DIFFERENTIATION

Project: Research project

Description

In previous work, we developed methods for the culture of
oligodendroglia, Schwann cells and astroglia, and were the first to
demonstrate: 1) a specific mechanism for adhesion of neuroglia
to axolemma; 2) that axolemma contact, though stimulating
Schwann cell mitosis, markedly inhibits proliferation of cultured
astroglia; and 3) that agents which elevate intracellular cyclic
adenosine 3', 5'-monophosphate (cyclic AMP) increase synthesis of
the myelin lipid galactocerebroside (galC) by both oligodendroglia
and Schwann cells. We will now study the process by which mature oligodendroglia
are derived from undifferentiated precursor cells. In the rat,
these precursor cells, which can develop into either
oligodendroglia or glial fibrillary acidic protein (GFAP) rich
astroglia, are identifiable by surface binding of a monoclonal
antibody, A2B5. We will purify and culture these precursor cells
and seek to identify phenotypic markers for them that, unlike
A2B5 binding, are related to function and applicable to species
other than the rat. We will determine effects on proliferation and
differentiation of these cells of activation of cyclic AMP
dependent protein kinase, of heparin-binding growth factors, and
of growth factors secreted by endothelial cells and astroglia. We have developed a clone of rapidly proliferating, phenotypically
stable peripheral neuroglia which synthesizes both a myelin-
specific glycoprotein (P/o) and GFAP. This clone will be used to
investigate how neuroglial precursor cells are guided to
differentiate into either a myelin-forming or a glial filament-rich
phenotype.
StatusFinished
Effective start/end date9/1/876/30/13

Funding

  • National Institutes of Health
  • National Institutes of Health: $275,625.00
  • National Institutes of Health: $352,468.00
  • National Institutes of Health: $309,528.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $345,488.00
  • National Institutes of Health: $296,734.00
  • National Institutes of Health
  • National Institutes of Health: $272,869.00
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health
  • National Institutes of Health: $307,900.00
  • National Institutes of Health
  • National Institutes of Health: $298,970.00
  • National Institutes of Health: $35,000.00
  • National Institutes of Health
  • National Institutes of Health: $288,141.00
  • National Institutes of Health: $270,113.00
  • National Institutes of Health
  • National Institutes of Health: $275,625.00
  • National Institutes of Health
  • National Institutes of Health: $303,037.00
  • National Institutes of Health: $173,250.00
  • National Institutes of Health: $50,000.00

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Oligodendroglia
Myelin Sheath
Neuroglia
Intercellular Signaling Peptides and Proteins
Schwann Cells
Glial Fibrillary Acidic Protein
Autoimmune Experimental Encephalomyelitis
Astrocytes
Clone Cells
Multiple Sclerosis
Fibroblast Growth Factor 2
P-Glycoprotein
D-Aspartic Acid
Mitosis
Protein Kinases
Glutamate Receptors
Heparin
Endothelial Cells
Platelet-Derived Growth Factor
Cell Proliferation

ASJC

  • Medicine(all)
  • Neuroscience(all)